The whitefly-associated facultative symbiont Hamiltonella defensa suppresses induced plant defences in tomato

doi:10.1111/1365-2435.12405

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	The whitefly-associated facultative symbiont Hamiltonella defensa suppresses induced plant defences in tomato
	Su, Qi 1,2; Oliver, Kerry M.3; Xie, Wen 1; Wu, Qingjun 1; Wang, Shaoli 1; Zhang, Youjun 1
刊名	FUNCTIONAL ECOLOGY
	2015
卷号	29 期号:8 页码:1007-1018
关键词	plant-insect interaction host-microbe endosymbiont salicylic acid jasmonic acid plant defence herbivory
ISSN号	0269-8463
DOI	10.1111/1365-2435.12405
通讯作者	Su, Qi
英文摘要	Maternally inherited bacterial symbionts are present in many, if not most, insect species. While there is rapidly accumulating evidence that facultative, heritable symbionts often protect insect hosts from natural enemies, there have been few clear examples where facultative symbionts mediate herbivore-plant interactions. The phloem-feeding whitefly, Bemisia tabaci, is a major agricultural pest that frequently harbours facultative symbionts, including Hamiltonella defensa. While H.defensa and other facultative symbionts have been shown to improve whitefly performance on particular food plants, no direct and specific roles for symbiont-mediated interactions with food plants have been identified. Here, we conducted a series of assays to determine whether infection with H.defensa improved whitefly performance on tomato (Solanum lycopersicum) and whether this benefit was associated with symbiont effects on induced plant defences. Finally, we tested whether impacts on induced defences involved in the modulation of a salivary factor. The downregulation of plant defences was associated with increased whitefly fecundity and survival. Feeding by H.defensa-infected whiteflies suppressed JA and JA-related anti-herbivore-induced defences in tomato relative to uninfected controls. That saliva-only treatments of damaged tomato leaves from H.defensa-infected whiteflies suppressed induced plant defences compared to saliva from uninfected controls, suggesting that elicitors in saliva were responsible. Characterization of the putative salivary factor(s) revealed they are likely small (< 3-kDa) and nonproteinaceous. Interestingly, suppression of defences was not observed in SA-deficient NahG plants, indicating that suppression of JA-regulated defences depends on the SA signalling pathway. This finding reveals an intriguing example of the crosstalk between SA and JA signalling pathways and suggests that infection with facultative symbionts can result in the manipulation of induced plant defences to the benefit of the insect host and heritable symbiont. Our results show that the bacterial symbiont H.defensa mediates whitefly-plant interactions by suppressing induced plant defences in tomato. This finding is among the first showing a direct role for facultative symbionts in mediating plant-herbivore interactions and demonstrates a novel tactic for insect herbivores to circumvent plant defences. Symbiont-mediated suppression of plant defences may enhance the deleterious effects of insect pests feeding on important crops.
学科主题	Ecology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000358948500003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100891]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China 2.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China 3.Univ Georgia, Dept Entomol, Athens, GA 30602 USA
推荐引用方式 GB/T 7714	Su, Qi,Oliver, Kerry M.,Xie, Wen,et al. The whitefly-associated facultative symbiont Hamiltonella defensa suppresses induced plant defences in tomato[J]. FUNCTIONAL ECOLOGY,2015,29(8):1007-1018.
APA	Su, Qi,Oliver, Kerry M.,Xie, Wen,Wu, Qingjun,Wang, Shaoli,&Zhang, Youjun.(2015).The whitefly-associated facultative symbiont Hamiltonella defensa suppresses induced plant defences in tomato.FUNCTIONAL ECOLOGY,29(8),1007-1018.
MLA	Su, Qi,et al."The whitefly-associated facultative symbiont Hamiltonella defensa suppresses induced plant defences in tomato".FUNCTIONAL ECOLOGY 29.8(2015):1007-1018.