Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp conglutinans in Cabbage (Brassica oleracea) Roots

doi:10.1371/journal.pone.0148048

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	Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp conglutinans in Cabbage (Brassica oleracea) Roots
	Xing, Miaomiao 1; Lv, Honghao 2; Ma, Jian 1; Xu, Donghui 2; Li, Hailong 1; Yang, Limei 2; Kang, Jungen 1; Wang, Xiaowu 2; Fang, Zhiyuan 2
刊名	PLOS ONE
	2016
卷号	11 期号:2 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0148048
通讯作者	Xing, Miaomiao
英文摘要	Fusarium wilt caused by Fusarium oxysporum f. sp. conglutinans (FOC) is a destructive disease of Brassica crops, which results in severe yield losses. There is little information available about the mechanism of disease resistance. To obtain an overview of the transcriptome profiles in roots of R4P1, a Brassica oleracea variety that is highly resistant to fusarium wilt, we compared the transcriptomes of samples inoculated with FOC and samples inoculated with distilled water. RNA-seq analysis generated more than 136 million 100-bp clean reads, which were assembled into 62,506 unigenes (mean size = 741 bp). Among them, 49,959 (79.92%) genes were identified based on sequence similarity searches, including SwissProt (29,050, 46.47%), Gene Ontology (GO) (33,767, 54.02%), Clusters of Orthologous Groups (KOG) (14,721, 23.55%) and Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG) (12,974, 20.76%) searches; digital gene expression analysis revealed 885 differentially expressed genes (DEGs) between infected and control samples at 4, 12, 24 and 48 hours after inoculation. The DEGs were assigned to 31 KEGG pathways. Early defense systems, including the MAPK signaling pathway, calcium signaling and salicylic acid-mediated hypersensitive response (SA-mediated HR) were activated after pathogen infection. SA-dependent systemic acquired resistance (SAR), ethylene (ET)- and jasmonic (JA)-mediated pathways and the lignin biosynthesis pathway play important roles in plant resistance. We also analyzed the expression of defense-related genes, such as genes encoding pathogenesis-related (PR) proteins, UDP-glycosyltransferase (UDPG), pleiotropic drug resistance, ATP-binding cassette transporters (PDR-ABC transporters), myrosinase, transcription factors and kinases, which were differentially expressed. The results of this study may contribute to efforts to identify and clone candidate genes associated with disease resistance and to uncover the molecular mechanism underlying FOC resistance in cabbage.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000369554000034
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100842]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops North, Beijing Vegetable Res Ctr, Minist Agr, 50 Zhanghua St, Beijing 100097, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr, 12 Zhongguancun Nandajie St, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Xing, Miaomiao,Lv, Honghao,Ma, Jian,et al. Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp conglutinans in Cabbage (Brassica oleracea) Roots[J]. PLOS ONE,2016,11(2):-.
APA	Xing, Miaomiao.,Lv, Honghao.,Ma, Jian.,Xu, Donghui.,Li, Hailong.,...&Fang, Zhiyuan.(2016).Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp conglutinans in Cabbage (Brassica oleracea) Roots.PLOS ONE,11(2),-.
MLA	Xing, Miaomiao,et al."Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp conglutinans in Cabbage (Brassica oleracea) Roots".PLOS ONE 11.2(2016):-.