苏云金芽孢杆菌cry9Aa基因的克隆,表达及活性分析

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	苏云金芽孢杆菌cry9Aa基因的克隆,表达及活性分析
	苏慧琴 1; 束长龙 2; 何康来 2; 张友军 3; 高继国 1; 宋福平 2
刊名	植物保护学报
	2010
卷号	37 期号:6 页码:541-546
关键词	苏云金芽孢杆菌 cry9Aa基因克隆生物活性
ISSN号	0577-7518
其他题名	Cloning,expression and insecticidal activity of two cry9Aa genes from Bacillus thuringiensis strains
英文摘要	为了解决目前广泛使用的cry1A类基因的抗性问题,获得对鳞翅目害虫高效杀虫基因,从菌株SC5D2和T03C001中鉴定并克隆到了2个新型cry9Aa基因,被Bt国际命名委员会分别正式命名为cry9Aa3和cry9Aa4,并进一步对其编码的蛋白进行活性测定.结果显示,2个基因对小菜蛾,玉米螟均具有较高的杀虫活性,其中对小菜蛾的LC50分别为1.83μg/mL和1.90μg/mL,对玉米螟的LC50分别为6.93μg/mL和1.06μg/mL.同时,两种Cry9Aa蛋白对Cry1Ac抗性小菜蛾都具有较高的杀虫活性,LC50分别为1.74μg/mL和1.39μg/mL,与敏感小菜蛾杀虫活性测定数据相当,表明Cry9Aa与Cry1Ac没有交互抗性; To solve the resistance risk problem of the widely applied cry1A gene and screen high toxic Lepidoptera specific cry genes,two cry9Aa genes from strain SC5D2 and T03C001 were identified and cloned respectively. The two genes were designated as cry9Aa3 and cry9Aa4 by International Nomenclature Committee of Bt Endotoxin. Furthermore,the toxicity of the two genes was evaluated. The results indicated that both the two toxins had high insecticidal activities against Plutella xylostella and Ostrinia furnacalis. The toxicity of the two toxins to P.xylostella with LC50 were 1.83 μg/mL and 1.90 μg/mL respectively while to O.furnacalis with LC50 were 6.93 μg/mL and 1.06 μg/mL. Also,the two toxins had high insecticidal activities against Cry1Ac-R P.xylostella strain with LC50 of 1.74 μg/mL and 1.39 μg/mL,respectively,similar to that against Cry1Ac-S P.xylostella strain. The results suggested that Cry9Aa toxin exhibited no cross-resistant to Cry1Ac toxin against P.xylostella
学科主题	植物学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100645]
专题	蔬菜花卉研究所_职能部门
作者单位	1.东北农业大学生命科学院, 哈尔滨, 150030 2.中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京, 100193 3.中国农业科学院蔬菜花卉研究所, 北京, 100081
推荐引用方式 GB/T 7714	苏慧琴,束长龙,何康来,等. 苏云金芽孢杆菌cry9Aa基因的克隆,表达及活性分析[J]. 植物保护学报,2010,37(6):541-546.
APA	苏慧琴,束长龙,何康来,张友军,高继国,&宋福平.(2010).苏云金芽孢杆菌cry9Aa基因的克隆,表达及活性分析.植物保护学报,37(6),541-546.
MLA	苏慧琴,et al."苏云金芽孢杆菌cry9Aa基因的克隆,表达及活性分析".植物保护学报 37.6(2010):541-546.