Plasma Membrane Intrinsic Proteins SlPIP2;1, SlPIP2;7 and SlPIP2;5 Conferring Enhanced Drought Stress Tolerance in Tomato

doi:10.1038/srep31814

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Plasma Membrane Intrinsic Proteins SlPIP2;1, SlPIP2;7 and SlPIP2;5 Conferring Enhanced Drought Stress Tolerance in Tomato
	Li, Ren 1,2; Wang, Jinfang 1; Li, Shuangtao 1; Zhang, Lei 1; Qi, Chuandong 1; Weeda, Sarah 3; Zhao, Bing 1; Ren, Shuxin 3; Guo, Yang-Dong 1
刊名	SCIENTIFIC REPORTS
	2016
卷号	6 页码:-
ISSN号	2045-2322
DOI	10.1038/srep31814
通讯作者	Li, Ren
英文摘要	The function of aquaporin (AQP) protein in transporting water is crucial for plants to survive in drought stress. With 47 homologues in tomato (Solanum lycopersicum) were reported, but the individual and integrated functions of aquaporins involved in drought response remains unclear. Here, three plasma membrane intrinsic protein genes, SlPIP2;1, SlPIP2;7 and SlPIP2;5, were identified as candidate aquaporins genes because of highly expressed in tomato roots. Assay on expression in Xenopus oocytes demonstrated that SlPIP2s protein displayed water channel activity and facilitated water transport into the cells. With real-time PCR and in situ hybridization analysis, SlPIP2s were considered to be involved in response to drought treatment. To test its function, transgenic Arabidopsis and tomato lines overexpressing SlPIP2;1, SlPIP2;7 or SlPIP2;5 were generated. Compared with wild type, the over-expression of SlPIP2;1, SlPIP2;7 or SlPIP2;5 transgenic Arabidopsis and tomato plants all showed significantly higher hydraulic conductivity levels and survival rates under both normal and drought conditions. Taken together, this study concludes that aquaporins (SlPIP2;1, SlPIP2;7 and SlPIP2;5) contribute substantially to root water uptake in tomato plants through improving plant water content and maintaining osmotic balance.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000381704700001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105913]
专题	蔬菜花卉研究所
作者单位	1.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 3.Virginia State Univ, Sch Agr, POB 9061, Petersburg, VA 23806 USA
推荐引用方式 GB/T 7714	Li, Ren,Wang, Jinfang,Li, Shuangtao,et al. Plasma Membrane Intrinsic Proteins SlPIP2;1, SlPIP2;7 and SlPIP2;5 Conferring Enhanced Drought Stress Tolerance in Tomato[J]. SCIENTIFIC REPORTS,2016,6:-.
APA	Li, Ren.,Wang, Jinfang.,Li, Shuangtao.,Zhang, Lei.,Qi, Chuandong.,...&Guo, Yang-Dong.(2016).Plasma Membrane Intrinsic Proteins SlPIP2;1, SlPIP2;7 and SlPIP2;5 Conferring Enhanced Drought Stress Tolerance in Tomato.SCIENTIFIC REPORTS,6,-.
MLA	Li, Ren,et al."Plasma Membrane Intrinsic Proteins SlPIP2;1, SlPIP2;7 and SlPIP2;5 Conferring Enhanced Drought Stress Tolerance in Tomato".SCIENTIFIC REPORTS 6(2016):-.