Genetic Diversity of Radish (Raphanus sativus L.) Germplasm Resources Revealed by AFLP and RAPD Markers

doi:10.1007/s11105-010-0228-7

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Genetic Diversity of Radish (Raphanus sativus L.) Germplasm Resources Revealed by AFLP and RAPD Markers
	Kong, Qiusheng 1,2; Li, Xixiang 1; Xiang, Changping 2; Wang, Haiping 1; Song, Jiangping 1; Zhi, Haiying 1
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2011
卷号	29 期号:1 页码:217-223
关键词	Raphanus sativus Genetic diversity AFLP RAPD
ISSN号	0735-9640
DOI	10.1007/s11105-010-0228-7
通讯作者	Kong, Qiusheng
英文摘要	Genetic diversity of 56 radish accessions, representing nearly all the typical types and origins of cultivated radish germplasms conserved in the National Mid-term Genebank for Vegetables of China, was assessed with amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers. A total of 72 and 128 polymorphic bands were generated by the 12 selected RAPD primers and eight AFLP primer combinations respectively. A moderate correlation with the value of r = 0.66 was observed between AFLP and RAPD markers. The total 200 polymorphic bands were integrated to assess the genetic diversity of 56 radish accessions. The Jaccard similarity coefficients between the accessions varied from 0.30 to 0.83 with the mean of 0.54. Cluster analysis classified the germplasms into three groups of var. hortensis Becker, var. sativus, and var. niger Kerner. The three-dimensions scatter plot of principle coordinate analysis (PCA) further divided var. hortensis Becker germplasms into two separate groups. The results indicated that the genetic diversity harbored among var. hortensis Becker germplasms was very abundant, which could be further exploited for radish genetic improvement.
学科主题	Biochemical Research Methods ; Plant Sciences ; BIOCHEMICAL RESEARCH METHODS ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000286674600022
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101963]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 10086, Peoples R China 2.Huazhong Agr Univ, Coll Hort & Forestry, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Kong, Qiusheng,Li, Xixiang,Xiang, Changping,et al. Genetic Diversity of Radish (Raphanus sativus L.) Germplasm Resources Revealed by AFLP and RAPD Markers[J]. PLANT MOLECULAR BIOLOGY REPORTER,2011,29(1):217-223.
APA	Kong, Qiusheng,Li, Xixiang,Xiang, Changping,Wang, Haiping,Song, Jiangping,&Zhi, Haiying.(2011).Genetic Diversity of Radish (Raphanus sativus L.) Germplasm Resources Revealed by AFLP and RAPD Markers.PLANT MOLECULAR BIOLOGY REPORTER,29(1),217-223.
MLA	Kong, Qiusheng,et al."Genetic Diversity of Radish (Raphanus sativus L.) Germplasm Resources Revealed by AFLP and RAPD Markers".PLANT MOLECULAR BIOLOGY REPORTER 29.1(2011):217-223.