Functional stacking of three resistance genes against Phytophthora infestans in potato

doi:10.1007/s11248-011-9510-1

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	Functional stacking of three resistance genes against Phytophthora infestans in potato
	Zhu, Suxian 1; Li, Ying 2; Vossen, Jack H.1; Visser, Richard G. F.1; Jacobsen, Evert 1
刊名	TRANSGENIC RESEARCH
	2012
卷号	21 期号:1 页码:89-99
关键词	Potato Gene stacking Durable resistance Functionality
ISSN号	0962-8819
DOI	10.1007/s11248-011-9510-1
通讯作者	Zhu, Suxian
英文摘要	Functional stacking of broad spectrum resistance (R) genes could potentially be an effective strategy for more durable disease resistance, for example, to potato late blight caused by Phytophthora infestans (Pi). For this reason, three broad spectrum potato R genes (Rpi), Rpi-sto1 (Solanum stoloniferum), Rpi-vnt1.1 (S. venturii) and Rpi-blb3 (S. bulbocastanum) were selected, combined into a single binary vector pBINPLUS and transformed into the susceptible cultivar Desiree. Among the 550 kanamycin resistant regenerants, 28 were further investigated by gene specific PCRs. All regenerants were positive for the nptII gene and 23 of them contained the three Rpi genes, referred to as triple Rpi gene transformants. Detached leaf assay and agroinfiltration of avirulence (Avr) genes showed that the 23 triple Rpi gene transformants were resistant to the selected isolates and showed HR with the three Avr effectors indicating functional stacking of all the three Rpi genes. It is concluded that Avr genes, corresponding to the R genes to be stacked, must be available in order to assay for functionality of each stack component. No indications were found for silencing or any other negative effects affecting the function of the inserted Rpi genes. The resistance spectrum of these 23 triple Rpi gene transformants was, as expected, a sum of the spectra from the three individual Rpi genes. This is the first example of a one-step approach for the simultaneous domestication of three natural R genes against a single disease by genetic transformation.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; BIOCHEMICAL RESEARCH METHODS ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000302268700007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101931]
专题	蔬菜花卉研究所
作者单位	1.Univ Wageningen & Res Ctr, Wageningen UR Plant Breeding, NL-6708 PB Wageningen, Netherlands 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Suxian,Li, Ying,Vossen, Jack H.,et al. Functional stacking of three resistance genes against Phytophthora infestans in potato[J]. TRANSGENIC RESEARCH,2012,21(1):89-99.
APA	Zhu, Suxian,Li, Ying,Vossen, Jack H.,Visser, Richard G. F.,&Jacobsen, Evert.(2012).Functional stacking of three resistance genes against Phytophthora infestans in potato.TRANSGENIC RESEARCH,21(1),89-99.
MLA	Zhu, Suxian,et al."Functional stacking of three resistance genes against Phytophthora infestans in potato".TRANSGENIC RESEARCH 21.1(2012):89-99.