Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato

doi:10.1007/s00122-013-2162-1

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	Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato
	Zhang, Chunzhi 1; Liu, Lei 1; Zheng, Zheng 1; Sun, Yuyan 1; Zhou, Longxi 1; Yang, Yuhong 1; Cheng, Feng 1; Zhang, Zhonghua 1; Wang, Xiaowu 1; Huang, Sanwen 1
刊名	THEORETICAL AND APPLIED GENETICS
	2013
卷号	126 期号:10 页码:2643-2653
ISSN号	0040-5752
DOI	10.1007/s00122-013-2162-1
通讯作者	Zhang, Chunzhi
英文摘要	Late blight, caused by the oomycete pathogen Phytophthora infestans (Mont.) de Bary, is a devastating disease for tomato and potato crops. In the past decades, many late blight resistance (R) genes have been characterized in potato. In contrast, less work has been conducted on tomato. The Ph-3 gene from Solanum pimpinellifolium was introgressed into cultivated tomatoes and conferred broad-spectrum resistance to P. infestans. It was previously assigned to the long arm of chromosome 9. In this study, a high-resolution genetic map covering the Ph-3 locus was constructed using an F-2 population of a cross between Solanum lycopersicum CLN2037B (containing Ph-3) and S. lycopersicum LA4084. Ph-3 was mapped in a 0.5 cM interval between two markers, Indel_3 and P55. Eight putative genes were found in the corresponding 74 kb region of the tomato Heinz1706 reference genome. Four of these genes are resistance gene analogs (RGAs) with a typical nucleotide-binding adaptor shared by APAF-1, R proteins, and CED-4 domain. Each RGA showed high homology to the late blight R gene Rpi-vnt1.1 from Solanum venturii. Transient gene silencing indicated that a member of this RGA family is required for Ph-3-mediated resistance to late blight in tomato. Furthermore, this RGA family was also found in the potato genome, but the number of the RGAs was higher than in tomato.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000324873400016
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101876]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Univ Wageningen & Res Ctr, Wageningen UR Plant Breeding, NL-6708 PB Wageningen, Netherlands
推荐引用方式 GB/T 7714	Zhang, Chunzhi,Liu, Lei,Zheng, Zheng,et al. Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato[J]. THEORETICAL AND APPLIED GENETICS,2013,126(10):2643-2653.
APA	Zhang, Chunzhi.,Liu, Lei.,Zheng, Zheng.,Sun, Yuyan.,Zhou, Longxi.,...&Li, Junming.(2013).Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato.THEORETICAL AND APPLIED GENETICS,126(10),2643-2653.
MLA	Zhang, Chunzhi,et al."Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato".THEORETICAL AND APPLIED GENETICS 126.10(2013):2643-2653.