Neonicotinoid resistance and cDNA sequences of nicotinic acetylcholine receptor subunits of the western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae)

doi:10.1007/s13355-013-0212-4

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	Neonicotinoid resistance and cDNA sequences of nicotinic acetylcholine receptor subunits of the western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae)
	Minakuchi, Chieka 1; Inano, Yurika 1; Shi, Xueyan 2; Song, Dunlun 2; Zhang, Youjun 3; Miura, Ken 1; Miyata, Tadashi 1; Gao, Xiwu 2; Tanaka, Toshiharu 1; Sonoda, Shoji 4
刊名	APPLIED ENTOMOLOGY AND ZOOLOGY
	2013
卷号	48 期号:4 页码:507-513
关键词	Frankliniella occidentalis Neonicotinoids Nicotinic acetylcholine receptor Piperonyl butoxide
ISSN号	0003-6862
DOI	10.1007/s13355-013-0212-4
通讯作者	Minakuchi, Chieka
英文摘要	The western flower thrips, Frankliniella occidentalis (Pergande), is difficult to control because of high insecticide resistance. In this study, susceptibility to major insecticides was examined in two Japanese strains (H-1 and KC) and a Chinese strain (BJ) using a leaf-dipping method. All three strains were resistant to permethrin and acetamiprid at agriculturally recommended doses. The median lethal concentration (LC50) for acetamiprid was 1720 ppm in strain H-1, 4780 ppm in strain KC and > 6680 ppm in strain BJ. In the presence of piperonyl butoxide, an inhibitor of cytochrome P450 monooxygenases, the LC50 for acetamiprid was 312 ppm in strain H-1, 837 ppm in strain KC and 1250 ppm in strain BJ. These results suggested that metabolism by cytochrome P450 monooxygenases is involved in acetamiprid resistance in these strains, though other factors also seem to play a role. Furthermore, cDNA cloning of the nicotinic acetylcholine receptor (nAChR) subunits was performed using degenerate primers, and the presence or absence of a point mutation in nAChR beta 1 was confirmed. The R81T mutation that had been reported in Myzus persicae (Sulzer) nAChR beta 1 was not found in F. occidentalis strains tested in this study.
学科主题	Entomology ; ENTOMOLOGY
语种	英语
出版者	SPRINGER JAPAN KK
WOS记录号	WOS:000326393400012
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101868]
专题	蔬菜花卉研究所
作者单位	1.Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan 2.China Agr Univ, Dept Entomol, Beijing 100094, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 4.Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, Japan
推荐引用方式 GB/T 7714	Minakuchi, Chieka,Inano, Yurika,Shi, Xueyan,et al. Neonicotinoid resistance and cDNA sequences of nicotinic acetylcholine receptor subunits of the western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae)[J]. APPLIED ENTOMOLOGY AND ZOOLOGY,2013,48(4):507-513.
APA	Minakuchi, Chieka.,Inano, Yurika.,Shi, Xueyan.,Song, Dunlun.,Zhang, Youjun.,...&Sonoda, Shoji.(2013).Neonicotinoid resistance and cDNA sequences of nicotinic acetylcholine receptor subunits of the western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae).APPLIED ENTOMOLOGY AND ZOOLOGY,48(4),507-513.
MLA	Minakuchi, Chieka,et al."Neonicotinoid resistance and cDNA sequences of nicotinic acetylcholine receptor subunits of the western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae)".APPLIED ENTOMOLOGY AND ZOOLOGY 48.4(2013):507-513.