NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans

doi:10.1016/S2095-3119(14)60759-0

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	NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans
	Zhang Kun; Xu Jian-fei; Duan Shao-guang; Pang Wan-fu; Bian Chun-song; Liu Jie; Jin Li-ping
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2014
卷号	13 期号:8 页码:1662-1671
关键词	potato late blight resistance gene NBS profiling broad-spectrum resistance
ISSN号	2095-3119
DOI	10.1016/S2095-3119(14)60759-0
通讯作者	Zhang Kun
英文摘要	Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is the most serious disease of potato worldwide. The adoption of varieties with resistance genes, especially broad-spectrum resistance genes, is the most efficient approach to control late blight. Solanum demissum is a well-known wild potato species from which 11 race-specific resistance genes have been identified, however, no broad-spectrum resistance genes like RB have been reported in this species. Here, we report a novel reisistance locus from S. demissum that potentially confer broad-spectrum resistance to late blight. A small segregating population of S. demissum were assessed for resistance to aggressive P. infestans isolates (race 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11). This coupled with nucleotide binding site (NBS) profiling analyses, led to the identification of three fragments that linked to the potential candidate resistance gene(s). Cloning and sequence analysis of these fragments suggested that the identified resistance gene locus is located in the region containing R2 resistance gene at chromosome 4. Based on the sequences of the cloned fragments, a co-segregating sequence characterized amplified region (SCAR) marker, RDSP, was developed. The newly identified marker RDSP will be useful for marker assisted breeding and further cloning of this potential resistance gene locus.
学科主题	Agriculture, Multidisciplinary ; AGRICULTURE, MULTIDISCIPLINARY
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000340350000006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101856]
专题	蔬菜花卉研究所
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Tuber & Root Cro, Minist Agr, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang Kun,Xu Jian-fei,Duan Shao-guang,et al. NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2014,13(8):1662-1671.
APA	Zhang Kun.,Xu Jian-fei.,Duan Shao-guang.,Pang Wan-fu.,Bian Chun-song.,...&Jin Li-ping.(2014).NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans.JOURNAL OF INTEGRATIVE AGRICULTURE,13(8),1662-1671.
MLA	Zhang Kun,et al."NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans".JOURNAL OF INTEGRATIVE AGRICULTURE 13.8(2014):1662-1671.