Single Nucleus Genome Sequencing Reveals High Similarity among Nuclei of an Endomycorrhizal Fungus

doi:10.1371/journal.pgen.1004078

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Single Nucleus Genome Sequencing Reveals High Similarity among Nuclei of an Endomycorrhizal Fungus
	Lin, Kui 1; Limpens, Erik 2; Zhang, Zhonghua 3; Ivanov, Sergey 2; Saunders, Diane G. O.4; Mu, Desheng 5; Pang, Erli 1; Cao, Huifen 1; Cha, Hwangho 1; Lin, Tao 3
刊名	PLOS GENETICS
	2014
卷号	10 期号:1 页码:-
ISSN号	1553-7404
DOI	10.1371/journal.pgen.1004078
通讯作者	Lin, Kui
英文摘要	Nuclei of arbuscular endomycorrhizal fungi have been described as highly diverse due to their asexual nature and absence of a single cell stage with only one nucleus. This has raised fundamental questions concerning speciation, selection and transmission of the genetic make-up to next generations. Although this concept has become textbook knowledge, it is only based on studying a few loci, including 45S rDNA. To provide a more comprehensive insight into the genetic makeup of arbuscular endomycorrhizal fungi, we applied de novo genome sequencing of individual nuclei of Rhizophagus irregularis. This revealed a surprisingly low level of polymorphism between nuclei. In contrast, within a nucleus, the 45S rDNA repeat unit turned out to be highly diverged. This finding demystifies a long-lasting hypothesis on the complex genetic makeup of arbuscular endomycorrhizal fungi. Subsequent genome assembly resulted in the first draft reference genome sequence of an arbuscular endomycorrhizal fungus. Its length is 141 Mbps, representing over 27,000 protein-coding gene models. We used the genomic sequence to reinvestigate the phylogenetic relationships of Rhizophagus irregularis with other fungal phyla. This unambiguously demonstrated that Glomeromycota are more closely related to Mucoromycotina than to its postulated sister Dikarya.
学科主题	Genetics & Heredity ; GENETICS & HEREDITY
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000336525000042
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101854]
专题	蔬菜花卉研究所
作者单位	1.Beijing Normal Univ, Lab Computat Mol Biol, Coll Life Sci, Beijing 100875, Peoples R China 2.Wageningen Univ, Mol Biol Lab, Dept Plant Sci, NL-6700 AP Wageningen, Netherlands 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr,Sino Dutch Joint Lab Hort Genom, Beijing 100193, Peoples R China 4.Sainsbury Lab, Norwich, Norfolk, England 5.Novome Biotech Inc, Beijing, Peoples R China 6.Wageningen Univ, Cell Biol Lab, Dept Plant Sci, NL-6700 AP Wageningen, Netherlands 7.Wageningen Univ, Genet Lab, Dept Plant Sci, NL-6700 AP Wageningen, Netherlands 8.King Saud Univ, Coll Sci, Riyadh, Saudi Arabia 9.Chinese Acad Agr Sci, Agr Genome Inst Shenzhen, Shenzhen, Peoples R China
推荐引用方式 GB/T 7714	Lin, Kui,Limpens, Erik,Zhang, Zhonghua,et al. Single Nucleus Genome Sequencing Reveals High Similarity among Nuclei of an Endomycorrhizal Fungus[J]. PLOS GENETICS,2014,10(1):-.
APA	Lin, Kui.,Limpens, Erik.,Zhang, Zhonghua.,Ivanov, Sergey.,Saunders, Diane G. O..,...&Huang, Sanwen.(2014).Single Nucleus Genome Sequencing Reveals High Similarity among Nuclei of an Endomycorrhizal Fungus.PLOS GENETICS,10(1),-.
MLA	Lin, Kui,et al."Single Nucleus Genome Sequencing Reveals High Similarity among Nuclei of an Endomycorrhizal Fungus".PLOS GENETICS 10.1(2014):-.