Profiling and identification of the metabolites of baicalin and study on their tissue distribution in rats by ultra-high-performance liquid chromatography with linear ion trap-Orbitrap mass spectrometer

doi:10.1016/j.jchromb.2015.01.018

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Profiling and identification of the metabolites of baicalin and study on their tissue distribution in rats by ultra-high-performance liquid chromatography with linear ion trap-Orbitrap mass spectrometer
	Zhang, Jiayu 1; Cai, Wei 2; Zhou, Yuan 3; Liu, Ying 1; Wu, Xiaodan 4; Li, Yun 2; Lu, Jianqiu 1; Qiao, Yanjiang 2
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2015
卷号	985 页码:91-102
关键词	Baicalin UPLC-ESI-MS/MS Metabolic profile Distribution Multiple data processing method
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2015.01.018
通讯作者	Zhang, Jiayu
英文摘要	Baicalin (baicalein 7-O-glucuronide), which is one of the major bioactive constituents isolated from Scutellariae Radix, possesses many biological activities, such as antiallergic, antioxidation, and anti-inflammatory activities. In the present study, an efficient strategy was established using ultra-high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometer (UPLC-LTQ-Orbitrap MS) to profile the in vivo metabolic fate of baicalin in rat plasma, urine, and various tissues. A combination of post-acquisition mining methods including extracted ion chromatogram (EIC) and multiple mass defect filters (MMDF) was adopted to identify the common and uncommon baicalin metabolites from the full mass scan data sets. Their structures were elucidated based on the accurate mass measurement, relevant drug biotransformation knowledge, the characteristic collision induced fragmentation pattern of baicalin metabolites, and bibliography data. Based on the proposed strategy, a total of 32 metabolites were observed and characterized. The corresponding reactions in vivo such as methylation, hydrolysis, hydroxylation, methoxylation, glucuronide conjugation, sulfate conjugation, and their composite reactions, were all discovered in the study. The results demonstrated that the rat liver and kidney are the most important organs for the baicalin metabolites presence. Six metabolites might play an important role in exerting pharmacological effects of baicalin in vivo. The newly discovered baicalin metabolites significantly expanded our understanding on its pharmacological effects, and could be targets for future studies on the important chemical constituents from herbal medicines. (C) 2015 Published by Elsevier B.V.
学科主题	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000350925800012
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101801]
专题	蔬菜花卉研究所
作者单位	1.Beijing Univ Chinese Med, Ctr Sci Expt, Beijing 100029, Peoples R China 2.Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing 100102, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 4.Beijing Univ Chinese Med, Sch Basic Med Sci, Dept Herbal Med, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jiayu,Cai, Wei,Zhou, Yuan,et al. Profiling and identification of the metabolites of baicalin and study on their tissue distribution in rats by ultra-high-performance liquid chromatography with linear ion trap-Orbitrap mass spectrometer[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2015,985:91-102.
APA	Zhang, Jiayu.,Cai, Wei.,Zhou, Yuan.,Liu, Ying.,Wu, Xiaodan.,...&Qiao, Yanjiang.(2015).Profiling and identification of the metabolites of baicalin and study on their tissue distribution in rats by ultra-high-performance liquid chromatography with linear ion trap-Orbitrap mass spectrometer.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,985,91-102.
MLA	Zhang, Jiayu,et al."Profiling and identification of the metabolites of baicalin and study on their tissue distribution in rats by ultra-high-performance liquid chromatography with linear ion trap-Orbitrap mass spectrometer".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 985(2015):91-102.