Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita | |
Yao, Yu-Rong1; Tian, Xue-Liang1,2; Shen, Bao-Ming1,3; Mao, Zhen-Chuan1; Chen, Guo-hua1; Xie, Bing-Yan1 | |
刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY |
2015 | |
卷号 | 31期号:4页码:549-556 |
关键词 | Acremonium implicatum Biocontrol Endophytic Fungus gfp Parasitic Root-knot nematode |
ISSN号 | 0959-3993 |
DOI | 10.1007/s11274-014-1781-2 |
通讯作者 | Yao, Yu-Rong |
英文摘要 | Acremonium implicatum is an endophytic fungus with biocontrol potential against Meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. To understand its mode of plant endophytism and opportunistic egg parasitism, GFP-tagged A. implicatum was constructed by PEG-mediated protoplast transformation. By laser scanning confocal microscopy (LSCM), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (Acr-1). Acr-1 could colonize epidermal tissue, cortical tissue, and xylem of roots and form a mutualistic symbiosis with tomato host plants. LSCM of Acr-1 infecting M. incognita eggs revealed that hyphae penetrated the shell and grew inside eggs to form trophic hyphae. A large number of hyphae enveloped parasitized eggs. In addition, the egg shell integrity was destroyed by fungal penetration. The percentage of egg parasitism was 33.8 %. There were no marked differences between the wild type and mutant in nematode second-stage juvenile mortality and egg hatching and in fungal control efficiency in a pot experiment. In conclusion, gfp-transformation did not change the nematicidal activity of A. implicatum and is a tool to examine the mode of plant endophytism and opportunistic egg parasitism of A. implicatum. |
学科主题 | Biotechnology & Applied Microbiology |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000351294200002 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/101798] |
专题 | 蔬菜花卉研究所 |
作者单位 | 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Henan Inst Sci & Technol, Coll Resources & Environm, Xinxiang 453003, Peoples R China 3.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China |
推荐引用方式 GB/T 7714 | Yao, Yu-Rong,Tian, Xue-Liang,Shen, Bao-Ming,et al. Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2015,31(4):549-556. |
APA | Yao, Yu-Rong,Tian, Xue-Liang,Shen, Bao-Ming,Mao, Zhen-Chuan,Chen, Guo-hua,&Xie, Bing-Yan.(2015).Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,31(4),549-556. |
MLA | Yao, Yu-Rong,et al."Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 31.4(2015):549-556. |
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