Purification and Identification of Two Antifungal Cyclic Peptides Produced by Bacillus amyloliquefaciens L-H15

doi:10.1007/s12010-015-1708-x

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Purification and Identification of Two Antifungal Cyclic Peptides Produced by Bacillus amyloliquefaciens L-H15
	Han, Yuzhu 1,3; Zhang, Bao 1; Shen, Qian 4; You, Chengzhen 1; Yu, Yaqiong 2; Li, Pinglan 1; Shang, Qingmao 2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2015
卷号	176 期号:8 页码:2202-2212
关键词	Bacillus amyloliquefaciens Biological control Antifungal compounds Cyclic peptides Mass spectrometry
ISSN号	0273-2289
DOI	10.1007/s12010-015-1708-x
通讯作者	Han, Yuzhu
英文摘要	Bacillus amyloliquefaciens L-H15 with broad spectrum antifungal activity was used as a biocontrol agent to suppress Fusarium oxysporum and other soil-borne fungal plant pathogens. Two antifungal fractions were isolated by bioactivity-guided reversed-phase high-performance liquid chromatography. The two compounds were identified by tandem Q-TOF mass spectroscopy as C15 Iturin A (1) and a novel cyclic peptide with a molecular weight of 852.4 Da (2). Both compounds showed good inhibitory activities against three plant fungal pathogens in cylinder-plate diffusion assay. To our best knowledge, this is the first report on a cyclic antifungal peptide with a molecular weight of 852.4 Da. The strong antifungal activity suggests that the B. amyloliquefaciens L-H15 and its bioactive components might provide an alternative resource for the biocontrol of plant diseases and sustainable agriculture.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	HUMANA PRESS INC
WOS记录号	WOS:000359432900009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101782]
专题	蔬菜花卉研究所
作者单位	1.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Hort Crops, Beijing 100081, Peoples R China 3.Southwest Univ, Dept Anim Sci, Chongqing 402460, Peoples R China 4.Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA
推荐引用方式 GB/T 7714	Han, Yuzhu,Zhang, Bao,Shen, Qian,et al. Purification and Identification of Two Antifungal Cyclic Peptides Produced by Bacillus amyloliquefaciens L-H15[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2015,176(8):2202-2212.
APA	Han, Yuzhu.,Zhang, Bao.,Shen, Qian.,You, Chengzhen.,Yu, Yaqiong.,...&Shang, Qingmao.(2015).Purification and Identification of Two Antifungal Cyclic Peptides Produced by Bacillus amyloliquefaciens L-H15.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,176(8),2202-2212.
MLA	Han, Yuzhu,et al."Purification and Identification of Two Antifungal Cyclic Peptides Produced by Bacillus amyloliquefaciens L-H15".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 176.8(2015):2202-2212.