Identification of candidate MLO powdery mildew susceptibility genes in cultivated Solanaceae and functional characterization of tobacco NtMLO1

doi:10.1007/s11248-015-9878-4

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Identification of candidate MLO powdery mildew susceptibility genes in cultivated Solanaceae and functional characterization of tobacco NtMLO1
	Appiano, Michela 1; Pavan, Stefano 2; Catalano, Domenico 3; Zheng, Zheng 4; Bracuto, Valentina 2; Lotti, Concetta 5; Visser, Richard G. F.1; Ricciardi, Luigi 2; Bai, Yuling 1
刊名	TRANSGENIC RESEARCH
	2015
卷号	24 期号:5 页码:847-858
关键词	MLO Solanaceae Powdery mildew Resistance Plant breeding
ISSN号	0962-8819
DOI	10.1007/s11248-015-9878-4
通讯作者	Appiano, Michela
英文摘要	Specific homologs of the plant Mildew Locus O (MLO) gene family act as susceptibility factors towards the powdery mildew (PM) fungal disease, causing significant economic losses in agricultural settings. Thus, in order to obtain PM resistant phenotypes, a general breeding strategy has been proposed, based on the selective inactivation of MLO susceptibility genes across cultivated species. In this study, PCR-based methodologies were used in order to isolate MLO genes from cultivated solanaceous crops that are hosts for PM fungi, namely eggplant, potato and tobacco, which were named SmMLO1, StMLO1 and NtMLO1, respectively. Based on phylogenetic analysis and sequence alignment, these genes were predicted to be orthologs of tomato SlMLO1 and pepper CaMLO2, previously shown to be required for PM pathogenesis. Full-length sequence of the tobacco homolog NtMLO1 was used for a heterologous transgenic complementation assay, resulting in its characterization as a PM susceptibility gene. The same assay showed that a single nucleotide change in a mutated NtMLO1 allele leads to complete gene loss-of-function. Results here presented, also including a complete overview of the tobacco and potato MLO gene families, are valuable to study MLO gene evolution in Solanaceae and for molecular breeding approaches aimed at introducing PM resistance using strategies of reverse genetics.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000361459200007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101773]
专题	蔬菜花卉研究所
作者单位	1.Wageningen Univ, Lab Plant Breeding, NL-6708 PB Wageningen, Netherlands 2.Univ Bari Aldo Moro, Sect Genet & Plant Breeding, Dept Plant Soil & Food Sci, I-70126 Bari, Italy 3.Italian Natl Res Council, Inst Biosci & Bioresources, I-70126 Bari, Italy 4.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 5.Univ Foggia, Dept Agroenvironm Sci Chem & Crop Protect, I-71100 Foggia, Italy
推荐引用方式 GB/T 7714	Appiano, Michela,Pavan, Stefano,Catalano, Domenico,et al. Identification of candidate MLO powdery mildew susceptibility genes in cultivated Solanaceae and functional characterization of tobacco NtMLO1[J]. TRANSGENIC RESEARCH,2015,24(5):847-858.
APA	Appiano, Michela.,Pavan, Stefano.,Catalano, Domenico.,Zheng, Zheng.,Bracuto, Valentina.,...&Bai, Yuling.(2015).Identification of candidate MLO powdery mildew susceptibility genes in cultivated Solanaceae and functional characterization of tobacco NtMLO1.TRANSGENIC RESEARCH,24(5),847-858.
MLA	Appiano, Michela,et al."Identification of candidate MLO powdery mildew susceptibility genes in cultivated Solanaceae and functional characterization of tobacco NtMLO1".TRANSGENIC RESEARCH 24.5(2015):847-858.