Integrated Genetic and Cytogenetic Map of the Cucumber Genome | |
Ren, Yi; Zhang, Zhonghua; Liu, Jinhua; Staub, Jack E.; Han, Yonghua; Cheng, Zhouchao; Li, Xuefeng; Lu, Jingyuan; Miao, Han; Kang, Houxiang | |
刊名 | PLOS ONE |
2009 | |
卷号 | 4期号:6页码:- |
ISSN号 | 1932-6203 |
DOI | 10.1371/journal.pone.0005795 |
通讯作者 | Ren, Yi |
英文摘要 | The Cucurbitaceae includes important crops such as cucumber, melon, watermelon, squash and pumpkin. However, few genetic and genomic resources are available for plant improvement. Some cucurbit species such as cucumber have a narrow genetic base, which impedes construction of saturated molecular linkage maps. We report herein the development of highly polymorphic simple sequence repeat (SSR) markers originated from whole genome shotgun sequencing and the subsequent construction of a high-density genetic linkage map. This map includes 995 SSRs in seven linkage groups which spans in total 573 cM, and defines similar to 680 recombination breakpoints with an average of 0.58 cM between two markers. These linkage groups were then assigned to seven corresponding chromosomes using fluorescent in situ hybridization (FISH). FISH assays also revealed a chromosomal inversion between Cucumis subspecies [C. sativus var. sativus L. and var. hardwickii (R.) Alef], which resulted in marker clustering on the genetic map. A quarter of the mapped markers showed relatively high polymorphism levels among 11 inbred lines of cucumber. Among the 995 markers, 49%, 26% and 22% were conserved in melon, watermelon and pumpkin, respectively. This map will facilitate whole genome sequencing, positional cloning, and molecular breeding in cucumber, and enable the integration of knowledge of gene and trait in cucurbits. |
学科主题 | Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES |
语种 | 英语 |
出版者 | PUBLIC LIBRARY SCIENCE |
WOS记录号 | WOS:000266716900005 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/101209] |
专题 | 蔬菜花卉研究所 |
推荐引用方式 GB/T 7714 | Ren, Yi,Zhang, Zhonghua,Liu, Jinhua,et al. Integrated Genetic and Cytogenetic Map of the Cucumber Genome[J]. PLOS ONE,2009,4(6):-. |
APA | Ren, Yi.,Zhang, Zhonghua.,Liu, Jinhua.,Staub, Jack E..,Han, Yonghua.,...&Huang, Sanwen.(2009).Integrated Genetic and Cytogenetic Map of the Cucumber Genome.PLOS ONE,4(6),-. |
MLA | Ren, Yi,et al."Integrated Genetic and Cytogenetic Map of the Cucumber Genome".PLOS ONE 4.6(2009):-. |
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