Mapping of clubroot (Plasmodiophora brassicae) resistance in canola (Brassica napus)

doi:10.1111/ppa.12422

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Mapping of clubroot (Plasmodiophora brassicae) resistance in canola (Brassica napus)
	Zhang, H.1,2; Feng, J.3; Hwang, S. -F.3; Strelkov, S. E.2; Falak, I.4; Huang, X.4; Sun, R.1
刊名	PLANT PATHOLOGY
	2016
卷号	65 期号:3 页码:435-440
关键词	bulked segregant analysis genetic markers linkage pathotype QTL
ISSN号	0032-0862
DOI	10.1111/ppa.12422
通讯作者	Zhang, H.
英文摘要	Clubroot disease, caused by Plasmodiophora brassicae, has become a major problem in the production of cruciferous crops worldwide. In this study, a population of 121 doubled haploid (DH) lines derived from a crossing between a resistant and a susceptible canola (Brassica napus) genotype was subjected to phenotypic and genotypic studies to determine the inheritance and location of the resistance gene(s). After inoculation with pathotype 3 of P.brassicae, the lines showed a 1:1 segregation ratio for resistance, indicating that resistance in this population is controlled by a single gene. Fifteen PCR-based markers that were known to be linked to clubroot resistance (CR) genes were screened against genomic DNA from parents and resistant and susceptible bulks. Marker GC1680, linked to the CR gene CRa, exhibited polymorphism between the parents and between the resistant and susceptible bulks. CRa target primers were used to amplify fragments from the two parents and the resultant sequences were compared. A high degree of sequence similarity was found between the parents in the nucleotide binding site domain of CRa. In contrast, sequence polymorphisms were detected in the leucine-rich repeat (LRR) domain. One pair of primers that amplify a band from the LRR region of the resistant parent but not the susceptible parent was used to screen the DH population. Amplicons were obtained from 60 of the 61 resistant lines and two of the 60 susceptible lines; thus, three recombinants were found. Based on these results, a resistance locus linked to CRa was found.
学科主题	Agronomy ; Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000372338200009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100829]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada 3.Alberta Agr & Rural Dev, Crop Diversificat Ctr North, Edmonton, AB T5Y 6H3, Canada 4.Pioneer Hibred Prod Ltd, 12111 Mississauga Rd, Caledon, ON L7C 1X1, Canada
推荐引用方式 GB/T 7714	Zhang, H.,Feng, J.,Hwang, S. -F.,et al. Mapping of clubroot (Plasmodiophora brassicae) resistance in canola (Brassica napus)[J]. PLANT PATHOLOGY,2016,65(3):435-440.
APA	Zhang, H..,Feng, J..,Hwang, S. -F..,Strelkov, S. E..,Falak, I..,...&Sun, R..(2016).Mapping of clubroot (Plasmodiophora brassicae) resistance in canola (Brassica napus).PLANT PATHOLOGY,65(3),435-440.
MLA	Zhang, H.,et al."Mapping of clubroot (Plasmodiophora brassicae) resistance in canola (Brassica napus)".PLANT PATHOLOGY 65.3(2016):435-440.