结球甘蓝相对于大白菜连锁群特异InDel 标记的建立及应用

CORC > 中国农业科学院 > 蔬菜花卉研究所

	结球甘蓝相对于大白菜连锁群特异InDel 标记的建立及应用
	朱东旭 1; 王彦华 1; 赵建军 1; 刘博 2; 李晓峰 1; 轩淑欣 1; 申书兴 1
刊名	园艺学报
	2014
卷号	41 期号:8 页码:1699-1706
关键词	大白菜结球甘蓝 InDel 标记易位系连锁群
ISSN号	0513-353X
其他题名	Establishment and Application of Specific InDel Markers on Different Linkage Groups of Cabbage Compared with Chinese Cabbage
英文摘要	以4个大白菜品种和4个结球甘蓝品种为试材,对位于芸薹属结球甘蓝C基因组9个连锁群的458个InDel标记进行PCR筛选,筛选出286个结球甘蓝相对于大白菜的特异InDel标记,其中位于 C01连锁群31个,C02连锁群45个,C03连锁群54个,C04连锁群24个,C05连锁群22个,C06连锁群20个,C07连锁群32个,C08连锁群34个,C09连锁群24个。利用结球甘蓝C02连锁群特异InDel标记对实验室创建的大白菜—结球甘蓝易位系自交后代进行了初步鉴定,明确了外源甘蓝染色体片段的物理位置在18 096 729~18 786 494 bp 之间的689 764 bp 区域内。; A total of 458 InDel markers derived from 9 linkage groups of cabbage were screened on four cabbage and four Chinese cabbage varieties by PCR amplification. The results showed that 286 InDel markers were specific on cabbage compared with Chinese cabbage,31 of them on linkage group C01,45 of them on linkage group C02,54 of them on linkage group C03,24 of them on linkage group C04,22 of them on linkage group C05,20 of them on linkage group C06,32 of them on linkage group C07,34 of them on linkage group C08,and 24 of them on linkage group C09. The inbred progeny of one Chinese cabbage-cabbage translocation line developed by our laboratory was identified preliminarily by specific InDel markers on linkage group C02. The physical location of exogenous cabbage chromosome fragment was in the 689 764 bp region from the 18 096 729 bp to 18 786 494 bp.
学科主题	园艺
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100585]
专题	蔬菜花卉研究所
作者单位	1.河北农业大学园艺学院, 河北, 保定, 071001 2.中国农业科学院蔬菜花卉研究所, 北京, 100081
推荐引用方式 GB/T 7714	朱东旭,王彦华,赵建军,等. 结球甘蓝相对于大白菜连锁群特异InDel 标记的建立及应用[J]. 园艺学报,2014,41(8):1699-1706.
APA	朱东旭.,王彦华.,赵建军.,刘博.,李晓峰.,...&申书兴.(2014).结球甘蓝相对于大白菜连锁群特异InDel 标记的建立及应用.园艺学报,41(8),1699-1706.
MLA	朱东旭,et al."结球甘蓝相对于大白菜连锁群特异InDel 标记的建立及应用".园艺学报 41.8(2014):1699-1706.