Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)

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	Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)
	Xu Liang 1; Jiang Qiu-wei 1; Wu Jian 2; Wang Yan 1; Gong Yi-qin 1; Wang Xian-li 1; Limera Cecilia 1; Liu Li-wang 1
刊名	Journal of Integrative Agriculture
	2014
卷号	13 期号:11 页码:2362-2369
关键词	Raphanus sativus L. molecular mapping downy mildew seedling-stage resistance marker assisted selection
ISSN号	2095-3119
英文摘要	Downy mildew (DM), caused by the fungus Peronospora parasitica, is a destructive disease of radish (Raphanus sativus L.) worldwide. Host resistance has been considered as an attractive and environmentally friendly approach to control the disease. However, the genetic mechanisms of resistance in radish to the pathogen remain unknown. To determine the inheritance of resistance to DM, F_1, F_2 and BC_1F_1 populations derived from reciprocal crosses between a resistant line NAU-dhp08 and a susceptible line NAU-qtbjq-06 were evaluated for their responses to DM at seedling stage. All F_1 hybrid plants showed high resistance to DM and maternal effect was not detected. The segregation for resistant to susceptible individuals statistically iftted a 3:1 ratio in two F_2 populations (F_(2(SR)) and F_(2(RS))), and 1:1 ratio in two BC_1F_1 populations, indicating that resistance to DM at seedling stage in radish was controlled by a single dominant locus designated as RsDmR. A total of 1 972 primer pairs (1 036 SRAP, 628 RAPD, 126 RGA, 110 EST-SSR and 72 ISSR) were screened, and 36 were polymorphic between the resistant and susceptible bulks, and consequently used for genotyping individuals in the F_2 population. Three markers (Em9/ga24_(370), NAUISSR826_(700) and Me7/em10_(400)) linked to the RsDmR locus within a 10.0 cM distance were identiifed using bulked segregant analysis (BSA). The SRAP marker Em9/ga24_(370) was the most tightly linked one with a distance of 2.3 cM to RsDmR. These markers tightly linked to the RsDmR locus would facilitate marker-assisted selection and resistance gene pyramiding in radish breeding programs.
学科主题	遗传学 ; 分子生物学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100580]
专题	蔬菜花卉研究所
作者单位	1.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Engn Res Ctr Hort Crop Germplasm Enhancement & Ut, Minist Educ,Coll Hort, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Xu Liang,Jiang Qiu-wei,Wu Jian,et al. Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)[J]. Journal of Integrative Agriculture,2014,13(11):2362-2369.
APA	Xu Liang.,Jiang Qiu-wei.,Wu Jian.,Wang Yan.,Gong Yi-qin.,...&Liu Li-wang.(2014).Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.).Journal of Integrative Agriculture,13(11),2362-2369.
MLA	Xu Liang,et al."Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)".Journal of Integrative Agriculture 13.11(2014):2362-2369.