Preparation and properties of abzyme with thyroxine deiodinase

	Preparation and properties of abzyme with thyroxine deiodinase
	Sun LW ; Liu LY ; Zhao DQ
刊名	chemical journal of chinese universities-chinese
	2002
卷号	23 期号:12 页码:2217-2220
关键词	I IODOTHYRONINE DEIODINASE 5'-DEIODINASE ANTIBODY ENZYME
ISSN号	0251-0790
通讯作者	zhao dq
中文摘要	the abzyme (se-6e8) with a higher thyroxine deiodinase activity was prepared by modifying the serine residues of monoclonal antibody (6e8)with phenylmethanesulfonyl fluoride and sodium hydrogen selenide, and the 6e8 against o-methyl-t-4, which is a kind of thyroxine derivatives and was taken as a hapten for the first time. two bands were found corresponding to the 5.5 kd heavy chain and the 2.7 kd light chain respectively by sds-page. the characteristics of dissociation constants, ph, and temperature were also studied. the results show that the activity of se-6e8 is 2 010 u/mumol protein, and the proper temperature and ph of the catalytic reactions is 57 degreesc and 8.2 respectively.
收录类别	SCI收录期刊论文
语种	中文
WOS记录号	WOS:000179776400001
公开日期	2010-11-03
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/18165]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Sun LW,Liu LY,Zhao DQ. Preparation and properties of abzyme with thyroxine deiodinase[J]. chemical journal of chinese universities-chinese,2002,23(12):2217-2220.
APA	Sun LW,Liu LY,&Zhao DQ.(2002).Preparation and properties of abzyme with thyroxine deiodinase.chemical journal of chinese universities-chinese,23(12),2217-2220.
MLA	Sun LW,et al."Preparation and properties of abzyme with thyroxine deiodinase".chemical journal of chinese universities-chinese 23.12(2002):2217-2220.