Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane

	Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane
	Huang WM ; Jia JB ; Zhang ZL ; Han XJ ; Tang JL ; Wang JG ; Dong SJ ; Wang EK
刊名	biosensors & bioelectronics
	2003
卷号	18 期号:10 页码:1225-1230
关键词	CYTOCHROME-C INFRARED-SPECTROSCOPY ELECTRON-TRANSFER BRAIN-TISSUE HEMOPROTEINS CHEMISTRY GOLD FILM ELECTROCHEMISTRY CARBON
ISSN号	0956-5663
通讯作者	wang ek
中文摘要	a highly catalytic activity microperoxidase-11 (mp-11) biosensor for h2o2 was developed to immobilizing the heme peptide in didodecyldimethylammonium bromide (ddab) lipid membrane. the enzyme electrode thus obtained responded to h2o2 without electron mediator or promoter, at a potential of +0.10 v versus ag \ agcl. a linear calibration curve is obtained over the range from 2.0 x 10(-5) to 2.4 x 10(-3) m. the biosensor responds to hydrogen peroxide in 15 s and has a detection limit of 8 x 10(-7) m (s/n = 3) providing a natural environment with lipid membrane for protein immobilization and maintenance of protein functions is a suitable option for the design of biosensors.
收录类别	SCI
语种	英语
WOS记录号	WOS:000184424600004
公开日期	2010-09-13
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/17517]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Huang WM,Jia JB,Zhang ZL,et al. Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane[J]. biosensors & bioelectronics,2003,18(10):1225-1230.
APA	Huang WM.,Jia JB.,Zhang ZL.,Han XJ.,Tang JL.,...&Wang EK.(2003).Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane.biosensors & bioelectronics,18(10),1225-1230.
MLA	Huang WM,et al."Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane".biosensors & bioelectronics 18.10(2003):1225-1230.