Electrochemical detection of DNA immobilized on gold colloid particles modified self-assembled monolayer electrode with silver nanoparticle label

	Electrochemical detection of DNA immobilized on gold colloid particles modified self-assembled monolayer electrode with silver nanoparticle label
	Wang MJ ; Sun CY ; Wang LY ; Ji XH ; Bai YB ; Li TJ ; Li JH
刊名	journal of pharmaceutical and biomedical analysis
	2003
卷号	33 期号:5 页码:1117-1125
关键词	STRANDED DEOXYRIBONUCLEIC-ACID HYBRIDIZATION DETECTION BIOSENSOR CARBON SENSOR VIRUS ASSAY SIZE
ISSN号	0731-7085
通讯作者	bai yb
中文摘要	the target dna was immobilized successfully on gold colloid particles associated with a cysteamine monolayer on gold electrode surface. self-assembly of colloidal an onto a cysteamine modified gold electrode can enlarge the electrode surface area and enhance greatly the amount of immobilized single stranded dna (ssdna). the electrontransfer processes of [fe(cn)(6)](4)-/[fe(cn)(6)](3-) on the gold surface were blocked due to the procedures of the target dna immobilization, which was investigated by impedance spectroscopy. then single stranded target dna immobilized on the gold electrode hybridized with the silver nanoparticle-oligonucleotide dna probe, followed by the release of the silver metal atoms anchored on the hybrids by oxidative metal dissolution, and the indirect determination of the released solubilized ag-1 ions by anodic stripping voltammetry (asv) at a carbon fiber microelectrode. the results show that this method has good correlation for dna detection in the range of 10-800 pmol/1 and allows the detection level as low as 5 pmol/1 of the target oligonucleotides.
收录类别	SCI
语种	英语
WOS记录号	WOS:000187951200026
公开日期	2010-09-13
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/17263]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang MJ,Sun CY,Wang LY,et al. Electrochemical detection of DNA immobilized on gold colloid particles modified self-assembled monolayer electrode with silver nanoparticle label[J]. journal of pharmaceutical and biomedical analysis,2003,33(5):1117-1125.
APA	Wang MJ.,Sun CY.,Wang LY.,Ji XH.,Bai YB.,...&Li JH.(2003).Electrochemical detection of DNA immobilized on gold colloid particles modified self-assembled monolayer electrode with silver nanoparticle label.journal of pharmaceutical and biomedical analysis,33(5),1117-1125.
MLA	Wang MJ,et al."Electrochemical detection of DNA immobilized on gold colloid particles modified self-assembled monolayer electrode with silver nanoparticle label".journal of pharmaceutical and biomedical analysis 33.5(2003):1117-1125.