Molecular Evidence of Barley Yellow Dwarf Virus Replication/  Movement Suppressed by the Resistance Gene Bdv2

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	Molecular Evidence of Barley Yellow Dwarf Virus Replication/ Movement Suppressed by the Resistance Gene Bdv2
	Liu Xiaodong; Zhang Zengyan; Liu Yan; Xin Zhiyong
刊名	遗传学报
	2005
卷号	32 期号:9 页码:942-947
关键词	barleybarley yellow dwarf virus semi-quantitative RT-PCR wheat-Thinopyrum intermedium translocation lines resistance
ISSN号	0379-4172
其他题名	抗病基因Bdv2抑制大麦黄矮病毒复制和运动的分子证据
英文摘要	A wheat-Thinopyrum intermedium translocation line YW642 possesses the resistance to GAV serotype of barley yellow dwarf virus （BYDV）, in which the resistance gene Bdv2 is derived from the chromosome 7X of Thinopyrum intermedium group 7. It is interesting to analyze BYDV accumulation content in the resistant and susceptible wheat plants for controlling BYDV disease and understanding the resistance mechanism against BYDV. In the paper, semi-quantitative reverse-transcription PCR （RT-PCR） was used to detect and quantify BYDV-GAV in the resistant and susceptible plants using specific primers for the coat protein （CP） and RNA-dependent RNA polymerase （RdRp） genes of BYDV-GAV serotype. On the inoculation site,the amount of the virus in the resistant wheat line （ YW642 ） was much lower compared to the susceptible sib line （ YW641 ）. There was small amount of the virus could be detected in YW642 at 2-5 clays post infestation （dpi）, afterwards the amount of virus decreased and no virus could be detected at 14 and 16 dpi. In the uninoculated upper leaves,no BYDV was detected in YW642 from 1 to 14 dpi,while the virus could be detected at 3 dpi and then accumulated rapidly in YW641. These results showed at molecular level that the replication and/or movement of BYDV-GAV were strongly suppressed in YW642 ,presumably owing to the action of the Bdv2 gene.
学科主题	分子生物学
语种	英语
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131320]
专题	作物科学研究所
作者单位	Institute of Crop Science, Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Genetics and Breeding Ministry of Agriculture, Beijing, 100081
推荐引用方式 GB/T 7714	Liu Xiaodong,Zhang Zengyan,Liu Yan,et al. Molecular Evidence of Barley Yellow Dwarf Virus Replication/ Movement Suppressed by the Resistance Gene Bdv2[J]. 遗传学报,2005,32(9):942-947.
APA	Liu Xiaodong,Zhang Zengyan,Liu Yan,&Xin Zhiyong.(2005).Molecular Evidence of Barley Yellow Dwarf Virus Replication/ Movement Suppressed by the Resistance Gene Bdv2.遗传学报,32(9),942-947.
MLA	Liu Xiaodong,et al."Molecular Evidence of Barley Yellow Dwarf Virus Replication/ Movement Suppressed by the Resistance Gene Bdv2".遗传学报 32.9(2005):942-947.