Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558

CORC > 中国农业科学院 > 作物科学研究所

	Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558
	Zheng Yongsheng 1; Ma Xiaogang 2; Chi Dezhao 2; Gao Ainong 1; Li Lihui 1; Liu Weihua 1
刊名	Journal of Integrative Agriculture
	2013
卷号	12 期号:3 页码:398-405
关键词	wheat spike-development inhibition 2-DE differentially expressed proteins
ISSN号	2095-3119
英文摘要	Spike number is one of three yield-related factors and is closely related to wheat yield. In the present study, we found that the inhibited and normal tillers of the 3558 line presented phenotypic differences at the elongation stage by morphological and anatomical analysis. We then initiated a proteomic study using two-dimensional electrophoresis (2-DE) and nanoscale liquid chromatography-high-definition tandem mass spectroscopy, to isolate and identify the key proteins and metabolic pathways related to spike-development inhibition. A total of 31 differentially expressed proteins (DEPs), which were mainly involved in cell cycle regulation, photosynthesis, glycolysis, stress response, and oxidation-reduction reactions, were isolated and identified. 14-3-3-like proteins and proliferating cell nuclear antigen (PCNA), involved in cell-cycle regulation, were dramatically down-regulated in inhibited tillers compared to normal tillers. Six spots corresponding to degraded Rubisco large subunits, involved in photosynthesis, were detected in different locations of the 2-DE gels and were up-regulated in inhibited tillers. In addition, the relative levels of DEPs involved in glycolysis and oxidationreduction reactions changed dramatically. Development was blocked or delayed at the elongation stage in the inhibited tillers of 3558. Weakened energy metabolism might be one reason that the inhibited tillers could not joint and develop into spikes. These DEPs and related metabolic pathways are significant for understanding the mechanism of spike-development inhibition and studying the spike-development process in wheat.
学科主题	农作物
语种	中文
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131203]
专题	作物科学研究所
作者单位	1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, 100081; 2.Qinghai Academy of Agriculture and Forestry, Xining, 810000
推荐引用方式 GB/T 7714	Zheng Yongsheng,Ma Xiaogang,Chi Dezhao,et al. Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558[J]. Journal of Integrative Agriculture,2013,12(3):398-405.
APA	Zheng Yongsheng,Ma Xiaogang,Chi Dezhao,Gao Ainong,Li Lihui,&Liu Weihua.(2013).Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558.Journal of Integrative Agriculture,12(3),398-405.
MLA	Zheng Yongsheng,et al."Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558".Journal of Integrative Agriculture 12.3(2013):398-405.