Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique

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	Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique
	Luo Meng; Kong Xiuying; Jiang Tao; Jia Chuan; Zhou Ronghua; Jia Jizeng
刊名	Acta Botanica Sinica
	2002
卷号	44 期号:5 页码:567-572
关键词	wheat Eiysiplie graminis expressed sequence tags disease resistance gene
ISSN号	1672-6650
其他题名	白粉病菌诱导的小麦表达序列标签（EST）研究
英文摘要	Powdery mildew is a serious disease of wheat in China. As part of ITEC (International Triteace EST cooperation), EST (expressed sequence tags) technique was used to explore the gene expression in leaf induced by Erysiphe graminls DC. A conventional cDNA library was constructed, and a total of 1 500 clones picked randomly from the library were sequenced, three hundred and eighty-seven ESTs of them were unique, which got the Accession Number in GenBank. About 49.4% ESTs showed significant similarity to functions of known sequences in GenBank. There are 196 ESTs' with functions not able to be determined, and eighty-four ESTs were demonstrated to be novel sequences. High-density dot membranes from unique clones were pro-duced, and several disease resistance related genes were screened by differential hybridization.
学科主题	植物学
语种	英语
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131159]
专题	作物科学研究所
作者单位	Institute of Crop Germplasm Resources, Chinese Academy of agricultural Sciences, Key Laboratory of Crop Germplasm and Biotechnology of Chinese Agriculture Ministry, CAS, 北京, 100081
推荐引用方式 GB/T 7714	Luo Meng,Kong Xiuying,Jiang Tao,et al. Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique[J]. Acta Botanica Sinica,2002,44(5):567-572.
APA	Luo Meng,Kong Xiuying,Jiang Tao,Jia Chuan,Zhou Ronghua,&Jia Jizeng.(2002).Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique.Acta Botanica Sinica,44(5),567-572.
MLA	Luo Meng,et al."Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique".Acta Botanica Sinica 44.5(2002):567-572.