Analyses of a Glycine max Degradome Library Identify microRNA Targets and MicroRNAs that Trigger Secondary SiRNA Biogenesis

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	Analyses of a Glycine max Degradome Library Identify microRNA Targets and MicroRNAs that Trigger Secondary SiRNA Biogenesis
	Hu Zheng 1; Jiang Qiyan 1; Ni Zhiyong 1; Chen Rui 2; Xu Shuo 1; Zhang Hui 1
刊名	Journal of Integrative Plant Biology
	2013
卷号	55 期号:2 页码:160-176
关键词	miRNAs miRNA targets secondary siRNAs degradome Glycine max
ISSN号	1672-9072
英文摘要	Plant microRNAs (miRNAs) regulate gene expression mainly by guiding cleavage of target mRNAs. In this study, a degradome library constructed from different soybean (Glycine max (L.) Merr.) tissues was deep-sequenced. 428 potential targets of small interfering RNAs and 25 novel miRNA families were identified. A total of 211 potential miRNA targets, including 174 conserved miRNA targets and 37 soybean-specific miRNA targets, were identified. Among them, 121 targets were first discovered in soybean. The signature distribution of soybean primary miRNAs (pri-miRNAs) showed that most pri-miRNAs had the characteristic pattern of Dicer processing. The biogenesis of TAS3 small interfering RNAs (siRNAs) was conserved in soybean, and nine Auxin Response Factors were identified as TAS3 siRNA targets. Twenty-three miRNA targets produced secondary small interfering RNAs (siRNAs) in soybean. These targets were guided by five miRNAs: gma-miR393, gma-miR1508, gma-miR1510, gma-miR1514, and novel-11. Multiple targets of these secondary siRNAs were detected. These 23 miRNA targets may be the putative novel TAS genes in soybean. Global identification of miRNA targets and potential novel TAS genes will contribute to research on the functions of miRNAs in soybean.
学科主题	植物学
语种	中文
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131157]
专题	作物科学研究所
作者单位	1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, The National Key Facilities for Crop Genetic Resources and Improvement, Beijing, 100081; 2.Central Laboratory of Tianjin Academy of Agricultural Sciences, Tianjin, 300381
推荐引用方式 GB/T 7714	Hu Zheng,Jiang Qiyan,Ni Zhiyong,et al. Analyses of a Glycine max Degradome Library Identify microRNA Targets and MicroRNAs that Trigger Secondary SiRNA Biogenesis[J]. Journal of Integrative Plant Biology,2013,55(2):160-176.
APA	Hu Zheng,Jiang Qiyan,Ni Zhiyong,Chen Rui,Xu Shuo,&Zhang Hui.(2013).Analyses of a Glycine max Degradome Library Identify microRNA Targets and MicroRNAs that Trigger Secondary SiRNA Biogenesis.Journal of Integrative Plant Biology,55(2),160-176.
MLA	Hu Zheng,et al."Analyses of a Glycine max Degradome Library Identify microRNA Targets and MicroRNAs that Trigger Secondary SiRNA Biogenesis".Journal of Integrative Plant Biology 55.2(2013):160-176.