Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil

doi:10.17221/43/2014-PPS

CORC > 中国农业科学院 > 植物保护研究所

	Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil
	Gao, Yu-Han 1; Miao, Wu 2; Guo, Rong-Jun 1; Li, Shi-Dong 1
刊名	PLANT PROTECTION SCIENCE
	2015
卷号	51 期号:2 页码:61-66
关键词	chilli root rot specific primer pair pathogen identification population detection population determination
ISSN号	1212-2580
DOI	10.17221/43/2014-PPS
通讯作者	Gao, Yu-Han
英文摘要	Root rot disease caused by Sclerotium rolfsii is one of the important factors limiting chilli production in southern China. Rapid methods of detection and identification of S. rolfsii are required for disease prediction and management. Herein, we adapted a four-step real time quantitative PCR method to detect S. rolfsii using new designed primer pair S301S/S301A. By using this assay, S. rolfsii was detected in ten soil samples and eight plant samples at concentrations as low as 10(-7) g mycelium/g soil or plant, both with and without typical symptoms, while tissue-culturing method could only detect the fungus in two samples. This rapid and quantitative method is efficient for detection and determination of S. rolfsii population in soil and plants.
学科主题	Agronomy ; Plant Sciences
语种	英语
出版者	CZECH ACADEMY AGRICULTURAL SCIENCES
WOS记录号	WOS:000354769600002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11810]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Pest Management Crops, Minist Agr, Beijing 100081, Peoples R China 2.Xiangyan Seed Co, Changsha, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yu-Han,Miao, Wu,Guo, Rong-Jun,et al. Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil[J]. PLANT PROTECTION SCIENCE,2015,51(2):61-66.
APA	Gao, Yu-Han,Miao, Wu,Guo, Rong-Jun,&Li, Shi-Dong.(2015).Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil.PLANT PROTECTION SCIENCE,51(2),61-66.
MLA	Gao, Yu-Han,et al."Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil".PLANT PROTECTION SCIENCE 51.2(2015):61-66.