Transcriptome differences between Cry1Ab resistant and susceptible strains of Asian corn borer

doi:10.1186/s12864-015-1362-2

CORC > 中国农业科学院 > 植物保护研究所

	Transcriptome differences between Cry1Ab resistant and susceptible strains of Asian corn borer
	Xu, Li-Na 1,2; Wang, Yue-Qin 2; Wang, Zhen-Ying 2; Hu, Ben-Jin 1; Ling, Ying-Hui 3; He, Kang-Lai 2
刊名	BMC GENOMICS
	2015
卷号	16 页码:-
关键词	Ostrinia furnacalis Cry1Ab Transcriptome differences Resistance Strains
ISSN号	1471-2164
DOI	10.1186/s12864-015-1362-2
通讯作者	Xu, Li-Na
英文摘要	Background: Asian corn borer (ACB), Ostrinia furnacalis (Guenee), is the major insect pest of maize in China and countries of East and Southeast Asia, the Pacific and Australasia. ACB can develop strong resistance to the transgenic Bt maize expressing Cry1Ab, the most widely commercialized Bt maize worldwide. However, the molecular basis for the resistance mechanisms of ACB to Cry1Ab remained unclear. Two biological replicates of the transcriptome of Bt susceptible (ACB-BtS) and Cry1Ab resistant (ACB-AbR) strains of ACB were sequenced using Solexa/Illumina RNA-Seq technology to identify Cry1Ab resistance-relevant genes.Results: The numbers of unigenes for two biological replications were 63,032 and 53,710 for ACB-BtS and 57,770 and 54,468 for ACB-AbR. There were 35,723 annotated unigenes from ACB reads found by BLAST searching NCBI non-redundant, NCBI non-redundant nucleotide, Swiss-prot protein, Kyoto Encyclopedia of Genes and Genomes, Cluster of Orthologous Groups of proteins, and Gene Ontology databases. Based on the NOISeq method, 3,793 unigenes were judged to be differentially expressed between ACB-BtS and ACB-AbR. Cry1Ab resistance appeared to be associated with change in the transcription level of enzymes involved in growth regulation, detoxification and metabolic/catabolic process. Among previously described Bt toxin receptors, the differentially expressed unigenes associated with aminopeptidase N and chymotrypsin/trypsin were up-regulated in ACB-AbR. Whereas, other putative Cry receptors, cadherin-like protein, alkaline phosphatase, glycolipid, actin, V-type proton ATPase vatalytic, heat shock protein, were under-transcripted. Finally, GPI-anchor biosynthesis was found to be involved in the significantly enriched pathway, and all genes mapped to the pathway were substantially down-regulated in ACB-AbR.Conclusion: To our knowledge, this is the first comparative transcriptome study to discover candidate genes involved in ACB Bt resistance. This study identified differentially expressed unigenes related to general Bt resistance in ACB. The assembled, annotated transcriptomes provides a valuable genomic resource for further understanding of the molecular basis of ACB Bt resistance mechanisms.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000353388300001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11754]
专题	植物保护研究所
作者单位	1.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230031, Anhui, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 3.Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Xu, Li-Na,Wang, Yue-Qin,Wang, Zhen-Ying,et al. Transcriptome differences between Cry1Ab resistant and susceptible strains of Asian corn borer[J]. BMC GENOMICS,2015,16:-.
APA	Xu, Li-Na,Wang, Yue-Qin,Wang, Zhen-Ying,Hu, Ben-Jin,Ling, Ying-Hui,&He, Kang-Lai.(2015).Transcriptome differences between Cry1Ab resistant and susceptible strains of Asian corn borer.BMC GENOMICS,16,-.
MLA	Xu, Li-Na,et al."Transcriptome differences between Cry1Ab resistant and susceptible strains of Asian corn borer".BMC GENOMICS 16(2015):-.