Determination of tumorigenic Agrobacterium density in soil by real-time PCR assay and its effect on crown gall disease severity

doi:10.1007/s10658-014-0586-3

CORC > 中国农业科学院 > 植物保护研究所

	Determination of tumorigenic Agrobacterium density in soil by real-time PCR assay and its effect on crown gall disease severity
	Li, Qian 1,2; Guo, Rong-Jun 2; Li, Shi-Dong 2; Li, Shi-Fang 2; Wang, Hong-Qing 1
刊名	EUROPEAN JOURNAL OF PLANT PATHOLOGY
	2015
卷号	142 期号:1 页码:25-36
关键词	Real-time PCR assay Tumorigenic Agrobacterium density Replanting soil Crown gall disease
ISSN号	0929-1873
DOI	10.1007/s10658-014-0586-3
通讯作者	Li, Qian
英文摘要	Tumorigenic Agrobacterium-induced crown gall disease significantly limits the production of peach, cherry, and other fruit trees in China. Thus, the rapid detection of pathogens is necessary for disease prediction and management. In this study, we designed the primer pair ipt 3F/ipt 3R on the basis of the ipt gene. This primer pair effectively amplified a 247 bp DNA fragment from the transferred DNA (T-DNA) sequences of tumorigenic Agrobacterium. By using this primer pair, we developed a sensitive real-time PCR method for quantifying tumorigenic Agrobacterium in soil at a level as low as 10(2) cfu/g. We used this assay to detect tumorigenic Agrobacterium in 19 soil samples. Levels were a parts per thousand currency sign10(2) cfu/g in maize or uncultivated soil and 10(3) to10(6) cfu/g in soil planting with peach or cherry. The relationship between the disease severity and the tumorigenic Agrobacterium density in soil was verified by artificial inoculation test. The disease incidence was 30 to 50 %, and the disease index was 20 to 30 in soil inoculated with A. tumefaciens at 10(3) cfu/g. The disease incidence increased to > 80 % when the inoculation density of A. tumefaciens was increased to > 10(5) cfu/g soil. These results indicated that tumorigenic Agrobacterium density in soil severely affected the incidence of crown gall formation on peach seedlings, and 10(3) bacterial cells in 1 g of soil were inappropriate for peach planting. Determination of pathogen density for gall formation can improve the prediction of crown gall disease outbreaks as well as aid in the management of this disease.
学科主题	Agronomy ; Plant Sciences ; Horticulture
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000351752300003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11722]
专题	植物保护研究所
作者单位	1.China Agr Univ, Coll Agr & Biotechnol, Dept Fruit Sci, Beijing 100193, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Pest Management Crops, Minist Agr, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Li, Qian,Guo, Rong-Jun,Li, Shi-Dong,et al. Determination of tumorigenic Agrobacterium density in soil by real-time PCR assay and its effect on crown gall disease severity[J]. EUROPEAN JOURNAL OF PLANT PATHOLOGY,2015,142(1):25-36.
APA	Li, Qian,Guo, Rong-Jun,Li, Shi-Dong,Li, Shi-Fang,&Wang, Hong-Qing.(2015).Determination of tumorigenic Agrobacterium density in soil by real-time PCR assay and its effect on crown gall disease severity.EUROPEAN JOURNAL OF PLANT PATHOLOGY,142(1),25-36.
MLA	Li, Qian,et al."Determination of tumorigenic Agrobacterium density in soil by real-time PCR assay and its effect on crown gall disease severity".EUROPEAN JOURNAL OF PLANT PATHOLOGY 142.1(2015):25-36.