Toxicological, Biochemical, and Histopathological Analyses Demonstrating That Cry1C and Cry2A Are Not Toxic to Larvae of the Honeybee, Apis mellifera

doi:10.1021/acs.jafc.5b01662

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	Toxicological, Biochemical, and Histopathological Analyses Demonstrating That Cry1C and Cry2A Are Not Toxic to Larvae of the Honeybee, Apis mellifera
	Wang, Yuan-Yuan 1; Li, Yun-He 1; Huang, Zachary Y.2; Chen, Xiu-Ping 1; Romeis, Joerg 1,3; Dai, Ping-Li 4; Peng, Yu-Fa 1
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2015
卷号	63 期号:27 页码:6126-6132
关键词	nontarget effects in vitro tier 1 study environmental risk assessment SBTI
ISSN号	0021-8561
DOI	10.1021/acs.jafc.5b01662
通讯作者	Wang, Yuan-Yuan
英文摘要	The honey bee, Apis mellifera, is commonly used as a test species for the regulatory risk assessment of insect-resistant genetically engineered (IRGE) plants. In the current study, a dietary exposure assay was developed, validated, and used to assess the potential toxicity of Cry1C and Cry2A proteins from Bacillus thuringiensis (Bt) to A. mellifera larvae; Cry1C and Cry2A are produced by different IRGE crops. The assay, Which uses the soybean trypsin inhibitor (SBTI) as a positive control and bovine serum: albumin (BSA) as a negative control, was used to measure the responses Of A. mellifera larvae to high concentrations of Cry1C and Cry2A. Survival was reduced and development was delayed when larvae were fed SBTI (1 mg/g diet) but were unaffected when larvae were fed BSA (400 mu g/g), Cry1C (50 mu g/g), or Cry2A (400 mu g/g). The enzymatic activities of A. mellifera larvae Were not altered and their midgut brush border membranes (BBMs) were not damaged after being fed with diets containing BSA, Cry1C, or Cry2A; however, enzymatic activities were increased and BBMs were damaged when diets contained SBTI. The study confirms that Cry1C and Cry2A have no acute toxicity to A mellifera larvae at concentrations >10 times higher than those detected in pollen from Bt plants.
学科主题	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000358186300004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11669]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA 3.Inst Sustainabil Sci ISS, Agroscope, CH-8046 Zurich, Switzerland 4.Chinese Acad Agr Sci, Inst Apicultural Res, Minist Agr, Key Lab Pollinating Insect Biol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yuan-Yuan,Li, Yun-He,Huang, Zachary Y.,et al. Toxicological, Biochemical, and Histopathological Analyses Demonstrating That Cry1C and Cry2A Are Not Toxic to Larvae of the Honeybee, Apis mellifera[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2015,63(27):6126-6132.
APA	Wang, Yuan-Yuan.,Li, Yun-He.,Huang, Zachary Y..,Chen, Xiu-Ping.,Romeis, Joerg.,...&Peng, Yu-Fa.(2015).Toxicological, Biochemical, and Histopathological Analyses Demonstrating That Cry1C and Cry2A Are Not Toxic to Larvae of the Honeybee, Apis mellifera.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,63(27),6126-6132.
MLA	Wang, Yuan-Yuan,et al."Toxicological, Biochemical, and Histopathological Analyses Demonstrating That Cry1C and Cry2A Are Not Toxic to Larvae of the Honeybee, Apis mellifera".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63.27(2015):6126-6132.