Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis

doi:10.1111/1744-7917.12181

CORC > 中国农业科学院 > 植物保护研究所

	Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis
	Li, Hui 1; Lang, Kun-Ling 1; Fu, Hai-Bin 2; Shen, Chang-Peng 1; Wan, Fang-Hao 1,3; Chu, Dong 1
刊名	INSECT SCIENCE
	2015
卷号	22 期号:6 页码:761-767
关键词	expressed sequence tags microsatellite marker normalized cDNA library Phenacoccus solenopsis
ISSN号	1672-9609
DOI	10.1111/1744-7917.12181
通讯作者	Li, Hui
英文摘要	The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.
学科主题	Entomology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000368270200006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11604]
专题	植物保护研究所
作者单位	1.Qingdao Agr Univ, Coll Agron & Plant Protect, Key Lab Integrated Crop Pest Management Shandong, Qingdao 266109, Shandong, Peoples R China 2.Shenyang Entry Exit Inspect & Quarantine Bur, Shenyang, Peoples R China 3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Li, Hui,Lang, Kun-Ling,Fu, Hai-Bin,et al. Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis[J]. INSECT SCIENCE,2015,22(6):761-767.
APA	Li, Hui,Lang, Kun-Ling,Fu, Hai-Bin,Shen, Chang-Peng,Wan, Fang-Hao,&Chu, Dong.(2015).Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis.INSECT SCIENCE,22(6),761-767.
MLA	Li, Hui,et al."Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis".INSECT SCIENCE 22.6(2015):761-767.