Identification and functional characterization of four transient receptor potential ankyrin 1 variants in Apolygus lucorum (Meyer-Dur)

doi:10.1111/imb.12231

CORC > 中国农业科学院 > 植物保护研究所

	Identification and functional characterization of four transient receptor potential ankyrin 1 variants in Apolygus lucorum (Meyer-Dur)
	Fu, T.1; Hull, J. J.2; Yang, T.1; Wang, G.1
刊名	INSECT MOLECULAR BIOLOGY
	2016
卷号	25 期号:4 页码:370-384
关键词	TRPA1 thermosensation mirid Apolygus lucorum chemosensation
ISSN号	0962-1075
DOI	10.1111/imb.12231
通讯作者	Fu, T.
英文摘要	As signal integrators that respond to various physical and chemical stimuli, transient receptor potential (TRP) channels fulfil critical functional roles in the sensory systems of both vertebrate and invertebrate organisms. Here, four variants of TRP ankyrin 1 (TRPA1) were identified and cloned from the green plant bug, Apolygus lucorum. Spatiotemporal expression profiling across development and in different adult tissues revealed that the highest relative-transcript levels occurred in first-instar nymphs and antennae, respectively. In Xenopus laevis-based functional assays, Apo. lucorum TRPA1-A (AlucTRPA1-A), AlucTRPA1-B and AlucTRPA1-C were activated by increasing the temperature from 20 to 40 degrees C with no significant desensitization observed after repeated temperature stimuli. The activation temperature of AlucTRPA1-A and AlucTRPA1-B was <25 degrees C, whereas the activation temperature of AlucTRPA1-C was between 25 and 30 degrees C. Amongst the variants, only AlucTRPA1-A and AlucTRPA1-C were directly activated by high concentrations of allyl isothiocyanate, cinnamaldehyde and citronellal. Taken together, these results suggest that AlucTRPA1 variants may function in vivo as both thermal and chemical sensors, with the four variants potentially mediating different physiological functions. This study not only enriches our understanding of TRPA1 function in Hemiptera (Miridae), but also offers a foundation for developing new pest control strategies.
学科主题	Biochemistry & Molecular Biology ; Entomology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000379613100004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11438]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.ARS, USDA, Arid Land Agr Ctr, Maricopa, AZ USA
推荐引用方式 GB/T 7714	Fu, T.,Hull, J. J.,Yang, T.,et al. Identification and functional characterization of four transient receptor potential ankyrin 1 variants in Apolygus lucorum (Meyer-Dur)[J]. INSECT MOLECULAR BIOLOGY,2016,25(4):370-384.
APA	Fu, T.,Hull, J. J.,Yang, T.,&Wang, G..(2016).Identification and functional characterization of four transient receptor potential ankyrin 1 variants in Apolygus lucorum (Meyer-Dur).INSECT MOLECULAR BIOLOGY,25(4),370-384.
MLA	Fu, T.,et al."Identification and functional characterization of four transient receptor potential ankyrin 1 variants in Apolygus lucorum (Meyer-Dur)".INSECT MOLECULAR BIOLOGY 25.4(2016):370-384.