Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis

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	Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis
	Yu, Hong-Kun 1; Chen, Hao 1; Zhang, Yong-Jun 1; Wu, Kong-Ming 1; Liang, Ge-Mei 1; Liu, Ze-Wen 2; Guo, Yu-Yuan 1
刊名	Insect Science
	2010
卷号	17 期号:5 页码:393-399
关键词	aminopeptidase N cadherin Chilo suppressalis heterologous expression Tn cells
ISSN号	1672-9609
英文摘要	The rice stem borer, Chilo suppressalis Walker is one of the most important insect pests on rice in Asia, north Africa and southern Europe. Transgenic Bt rice has been developed in the laboratory with good resistance to this pest and other Lepidopteran insects, which will provide a possible alternative tool for this pest control. The full-length cDNAs encoding an aminopeptidase N (CsAPN) and a cadherin (CsCad) were cloned from C. suppressalis. CsAPN showed common features of, and high identities to, other insect APNs in its deduced amino acid sequence. Although a full-length cDNA encoding cadherin-like protein has been reported in GenBank, the newly isolated cadherin here (CsCad) showed some differences in its amino acid sequence, especially at the 7th cadherin repeat region (CR7), which indicated the newly isolated CsCad might be another allele. CsAPN and CsCad were successfully expressed in insect Tn cells, and the blot analysis showed these two proteins could bind Bt toxin Cryl Ab. The results will provide valuable information for the studies of toxin mode of action and the possible toxin resistance mechanisms in this pest
学科主题	昆虫学
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11180]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China 2.Nanjing Agr Univ, Coll Plant Protect, Minist Agr, Key Lab Monitoring & Management Crop Dis & Pest I, Nanjing, Peoples R China
推荐引用方式 GB/T 7714	Yu, Hong-Kun,Chen, Hao,Zhang, Yong-Jun,et al. Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis[J]. Insect Science,2010,17(5):393-399.
APA	Yu, Hong-Kun.,Chen, Hao.,Zhang, Yong-Jun.,Wu, Kong-Ming.,Liang, Ge-Mei.,...&Guo, Yu-Yuan.(2010).Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis.Insect Science,17(5),393-399.
MLA	Yu, Hong-Kun,et al."Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis".Insect Science 17.5(2010):393-399.