The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

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	The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana
	Jiang Weiyu 1; Geng Lili 1; Dai Pingli 2; Lang Zhihong 3; Shu Changlong 1; Lin Yi 4; Zhou Ting 2; Song Fuping 1; Zhang Jie 1
刊名	Journal of Integrative Agriculture
	2013
卷号	12 期号:3 页码:474-482
关键词	Bacillus thuringiensis intestinal bacteria horizontal gene transfer transgenic maize Apis cerana Biosafety
ISSN号	2095-3119
英文摘要	Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-transgenic maize pollen, non-transgenic near isoline pollen, linear cry1Ah gene (800 ng mL~(-1)) and supercoiled plasmid DNA (800 ng mL~(-1)) under laboratory conditions. The DGGE profile showed that the number of DGGE bands varied from 10.7 to 14.7 per sample, and the Shannon’s index ranged from 0.85 to 1.00. The similarity calculated by PAST was mostly above 92%, indicating no obvious changes among treatments or within replicates. 14 bacterial strains affiliated with Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were isolated and characterized on media under aerobic and anaerobic conditions. These results demonstrated that transgenic cry1Ah maize pollen did not induce significant changes of the honeybee gut bacterial community composition under laboratory conditions.
学科主题	农作物 ; 畜牧、动物医学、狩猎、蚕、蜂
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11142]
专题	植物保护研究所
作者单位	1.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing, 100193 2.Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Beijing, 100093 3.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 4.College of Chemical Engineering, Huaqiao University, Xiamen, 361021
推荐引用方式 GB/T 7714	Jiang Weiyu,Geng Lili,Dai Pingli,et al. The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana[J]. Journal of Integrative Agriculture,2013,12(3):474-482.
APA	Jiang Weiyu.,Geng Lili.,Dai Pingli.,Lang Zhihong.,Shu Changlong.,...&Zhang Jie.(2013).The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana.Journal of Integrative Agriculture,12(3),474-482.
MLA	Jiang Weiyu,et al."The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana".Journal of Integrative Agriculture 12.3(2013):474-482.