Functional Characteristics of a Novel Chemosensory Protein in the Cotton Bollworm Helicoverpa armigera (Hubner)

CORC > 中国农业科学院 > 植物保护研究所

	Functional Characteristics of a Novel Chemosensory Protein in the Cotton Bollworm Helicoverpa armigera (Hubner)
	Zhang Tian-tao 1; Wang Wei-xuan 2; Zhang Zi-ding 2; Zhang Yong-jun 1; Guo Yu-yuan 1
刊名	Journal of Integrative Agriculture
	2013
卷号	12 期号:5 页码:853-861
关键词	Helicoverpa armigera chemosensory protein expression characteristics competitive binding assay 3D structure model
ISSN号	2095-3119
英文摘要	A chemosensory protein named HarmCSP5 in cotton bollworm Helicoverpa armigera(Hubner) was obtained from antennal cDNA libraries and expressed in Escherichia coli.The real time quantitative PCR(RT-qPCR) results indicated that HarmCSP5 gene was mainly expressed in male and female antennae but also expressed in female legs and wings.Competitive binding assays were performed to test the binding affinity of recombinant HarmCSP5 to 60 odor molecules including some cotton volatiles.The resules showed that HarmCSP5 showed strong binding abilities to 4-ehtylbenzaldehyde and 3,4-dimethlbenz aldehyde,whereas methyl phenylacetate,2-decanone,1-pentanol,carvenol,isoborneol,nerolidol,2nonanone and ethyl heptanoate have relatively weak binding affinity.Moreover,the predicted 3D model of HarmCSP5 consists of six α-helices located among residues 33-38(α1),40-48(α2),62-72(α3),80-96(α4),98-108(α5),and 116-119(α6),two pairs of disulfide bridges Cys49-Cys55,Cys75-Cys78.The two amino acid residues,Ile94 and Trp101,may play crucial roles in HarmCSP5 binding with ligands and need further study for confirmation.
学科主题	农作物
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11139]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Zhang Tian-tao,Wang Wei-xuan,Zhang Zi-ding,et al. Functional Characteristics of a Novel Chemosensory Protein in the Cotton Bollworm Helicoverpa armigera (Hubner)[J]. Journal of Integrative Agriculture,2013,12(5):853-861.
APA	Zhang Tian-tao,Wang Wei-xuan,Zhang Zi-ding,Zhang Yong-jun,&Guo Yu-yuan.(2013).Functional Characteristics of a Novel Chemosensory Protein in the Cotton Bollworm Helicoverpa armigera (Hubner).Journal of Integrative Agriculture,12(5),853-861.
MLA	Zhang Tian-tao,et al."Functional Characteristics of a Novel Chemosensory Protein in the Cotton Bollworm Helicoverpa armigera (Hubner)".Journal of Integrative Agriculture 12.5(2013):853-861.