Drug-human serum albumin binding studied by capillary electrophoresis with electrochemiluminescence detection

	Drug-human serum albumin binding studied by capillary electrophoresis with electrochemiluminescence detection
	Zhao XC ; You TY ; Liu JF ; Sun XH ; Yan JL ; Yang XR ; Wang EK
刊名	electrophoresis
	2004
卷号	25 期号:20 页码:3422-3426
关键词	RU(BPY)(3)(3+)-BASED CHEMILUMINESCENCE DETECTION ELECTROGENERATED CHEMILUMINESCENCE AMINO-ACIDS BIOPOLYMERS
ISSN号	0173-0835
通讯作者	wang ek
中文摘要	a new technique for investigating drug-protein binding was developed employing capillary electrophoresis (ce) coupled with tris(2,2'-bipyridyl) ruthenium(ii) [ru(bpy)(3)(2+)] electrochemiluminescence (ecl) (ce-ecl) detection after equilibrium dialysis. three basic drugs, namely pridinol, procyclidine and its analogue trihexyphenidyl, were successfully separated by capillary zone electrophoresis with end-column ru(bpy)(3)(2+) ecl detection. the relative drug binding to human serum albumin (hsa) for each single drug as well'as for the three drugs binding simultaneously was calculated. it was found that the three antiparkinsonian drugs compete for the same binding site on hsa. this work demonstrated that ru(bpy)(3)(2+) ce-ecl can be a suitable technique for studying drug-protein binding.
收录类别	SCI
语种	英语
WOS记录号	WOS:000224943700013
公开日期	2010-08-17
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/14801]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhao XC,You TY,Liu JF,et al. Drug-human serum albumin binding studied by capillary electrophoresis with electrochemiluminescence detection[J]. electrophoresis,2004,25(20):3422-3426.
APA	Zhao XC.,You TY.,Liu JF.,Sun XH.,Yan JL.,...&Wang EK.(2004).Drug-human serum albumin binding studied by capillary electrophoresis with electrochemiluminescence detection.electrophoresis,25(20),3422-3426.
MLA	Zhao XC,et al."Drug-human serum albumin binding studied by capillary electrophoresis with electrochemiluminescence detection".electrophoresis 25.20(2004):3422-3426.