Genetic transformation of wheat: current status and future prospects

doi:10.1007/s11816-011-0213-0

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	Genetic transformation of wheat: current status and future prospects
	Li, Jiarui 1,2; Ye, Xingguo 1; An, Baoyan 2; Du, Lipu 1; Xu, Huijun 1
刊名	PLANT BIOTECHNOLOGY REPORTS
	2012
卷号	6 期号:3 页码:183-193
关键词	Wheat Genetic transformation Techniques
ISSN号	1863-5466
DOI	10.1007/s11816-011-0213-0
通讯作者	Li, Jiarui
英文摘要	Genetic transformation is a reverse genetics tool for validation of target genes and crop improvement. However, due to its low efficiency and genotype dependency, wheat is considered a recalcitrant plant for genetic transformation. During the last 20 years, various in vitro and in planta transformation methods have been reported in wheat. Until now, biolistic particle and Agrobacterium-mediated wheat transformation methods using immature embryos as explants have been the two major transformation approaches. In addition to immature embryos, other explant types, such as mature embryos, anther-derived calli, inflorescences, apical meristems, and other floral organs, have been employed; however, they need further optimization. In addition to the common marker genes, such as bar, hpt and gus, other effective markers, ALS, AtMYB12 and pmi, have been successfully used for selection of positive transgenic plants. Numerous agronomic trait genes such as biotic stress resistance or tolerance genes have been transferred into wheat plants. Future prospects, such as recipient wheat cultivars and explants, marker free issues, and transgene silencing, are discussed. The objective of this review is to summarize current successful techniques for wheat transformation and stimulate further research into long-term wheat improvement by genetic engineering approaches.
学科主题	Biotechnology & Applied Microbiology ; Plant Sciences ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000306440000001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5149]
专题	作物科学研究所_其他01
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66502 USA
推荐引用方式 GB/T 7714	Li, Jiarui,Ye, Xingguo,An, Baoyan,et al. Genetic transformation of wheat: current status and future prospects[J]. PLANT BIOTECHNOLOGY REPORTS,2012,6(3):183-193.
APA	Li, Jiarui,Ye, Xingguo,An, Baoyan,Du, Lipu,&Xu, Huijun.(2012).Genetic transformation of wheat: current status and future prospects.PLANT BIOTECHNOLOGY REPORTS,6(3),183-193.
MLA	Li, Jiarui,et al."Genetic transformation of wheat: current status and future prospects".PLANT BIOTECHNOLOGY REPORTS 6.3(2012):183-193.