Characterization of the calcineurin B-Like (CBL) gene family in maize and functional analysis of ZmCBL9 under abscisic acid and abiotic stress treatments

doi:10.1016/j.plantsci.2016.09.011

	Characterization of the calcineurin B-Like (CBL) gene family in maize and functional analysis of ZmCBL9 under abscisic acid and abiotic stress treatments
	Zhang, Fan 1,2,3; Li, Li 1,2; Jiao, Zhenzhen 1,2; Chen, Yangsong 1,2; Liu, Hui 1,2; Chen, Xunji 1,2,4; Fu, Junjie 1,2; Wang, Guoying 1,2; Zheng, Jun 1,2
刊名	PLANT SCIENCE
	2016
卷号	253 页码:118-129
关键词	CBL Abscisic acid Abiotic stress Maize
ISSN号	0168-9452
DOI	10.1016/j.plantsci.2016.09.011
通讯作者	Zhang, Fan
英文摘要	In plants, calcineurin B-like proteins (CBLs) play crucial roles in regulating calcium-signaling in response to various abiotic stresses by interacting with specific CBL-interacting protein kinases (CIPKs). However, the identities and functions of CBL gene family members in maize are largely unknown. Here, we identified from the maize genome 12 CBL genes. All 12 CBLs have conserved EF-hand domains, and half harbor myristoylation motifs. We further characterized the function of one CBL gene, ZmCBL9, which can be induced by salt, dehydration, glucose and abscisic acid (ABA) treatments. Overexpression of ZmCBL9 enhanced resistance or tolerance to ABA, glucose, salt and osmotic stress in Arabidopsis and complemented the hypersensitive phenotype of the Arabidopsis cb19 mutant in response to ABA and abiotic stress. The ZmCBL9 gene negatively regulates the expression of genes in the ABA signaling, biosynthesis and catabolism pathways. Moreover, the ZmCBL9 protein is found to interact with eight maize CIPKs and these ZmCIPK genes were up-regulated by different stress treatments, including salt, dehydration, glucose, low potassium and ABA. These results suggest that ZmCBL9 may interact with various ZmCIPKs to regulate the abiotic stress and ABA response signaling in plants. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
学科主题	Plant Sciences
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000388049000013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105282]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Inst Bot, Jiangsu Prov, Nanjing 210014, Jiangsu, Peoples R China 3.Chinese Acad Sci, Nanjing 210014, Jiangsu, Peoples R China 4.Xinjiang Acad Agr Sci, Inst Nucl Technol & Biotechnol, Urumqi 830091, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Fan,Li, Li,Jiao, Zhenzhen,et al. Characterization of the calcineurin B-Like (CBL) gene family in maize and functional analysis of ZmCBL9 under abscisic acid and abiotic stress treatments[J]. PLANT SCIENCE,2016,253:118-129.
APA	Zhang, Fan.,Li, Li.,Jiao, Zhenzhen.,Chen, Yangsong.,Liu, Hui.,...&Zheng, Jun.(2016).Characterization of the calcineurin B-Like (CBL) gene family in maize and functional analysis of ZmCBL9 under abscisic acid and abiotic stress treatments.PLANT SCIENCE,253,118-129.
MLA	Zhang, Fan,et al."Characterization of the calcineurin B-Like (CBL) gene family in maize and functional analysis of ZmCBL9 under abscisic acid and abiotic stress treatments".PLANT SCIENCE 253(2016):118-129.