Cloning and characterization of a putative transcription factor induced by abiotic stress in Zea mays

	Cloning and characterization of a putative transcription factor induced by abiotic stress in Zea mays
	Jia, Zhiwei 1; Lian, Yun 1; Zhu, Yun 1; He, Junguang 1; Cao, Zuping 1; Wang, Guoying 2
刊名	AFRICAN JOURNAL OF BIOTECHNOLOGY
	2009
卷号	8 期号:24 页码:6764-6771
关键词	Maize ZmbZIP17 abiotic stress subcellular localization
ISSN号	1684-5315
通讯作者	Jia, Zhiwei
英文摘要	bZIP transcription factors have been reported to play important roles in plant responses to abiotic stresses. Here, we reported the cloning and characterization of a putative bZIP transcription factor (ZmbZIP17) from maize inbred line Han21, which is up-regulated by drought treatment. The open reading frame sequence of ZmbZIP17 was obtained by using 5'RACE and RT-PCR. Sequence analysis showed that ZmbZIP17 encodes a polypeptide of 563 amino acids with predicted molecular mass of 59.8 kDa and pI of 5.6. Southern blot analysis showed that ZmbZIP17 exists as a single copy gene in maize genome. Subcellular localization of ZmbZIP17 was identified in nucleus. The results of real-time PCR analysis indicated that ZmbZIP17 was up-regulated by drought, heat, ABA and NaCl stress immediately, which suggested that ZmbZIP17 is an early stage responsive gene to various abiotic stresses. The result also showed that ZmbZIP17 expressed much higher in leaves than in other organs in maize seedlings.
学科主题	Biotechnology & Applied Microbiology
语种	英语
出版者	ACADEMIC JOURNALS
WOS记录号	WOS:000273902200005
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5470]
专题	作物科学研究所_分子生物学系
作者单位	1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Jia, Zhiwei,Lian, Yun,Zhu, Yun,et al. Cloning and characterization of a putative transcription factor induced by abiotic stress in Zea mays[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(24):6764-6771.
APA	Jia, Zhiwei,Lian, Yun,Zhu, Yun,He, Junguang,Cao, Zuping,&Wang, Guoying.(2009).Cloning and characterization of a putative transcription factor induced by abiotic stress in Zea mays.AFRICAN JOURNAL OF BIOTECHNOLOGY,8(24),6764-6771.
MLA	Jia, Zhiwei,et al."Cloning and characterization of a putative transcription factor induced by abiotic stress in Zea mays".AFRICAN JOURNAL OF BIOTECHNOLOGY 8.24(2009):6764-6771.