TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis

doi:10.1093/jxb/erp331

	TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis
	Mao, Xinguo 1; Zhang, Hongying 1,2; Tian, Shanjun 1,3; Chang, Xiaoping 1; Jing, Ruilian 1
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2010
卷号	61 期号:3 页码:683-696
关键词	Abiotic stress morphological character physiological trait stress responses
ISSN号	0022-0957
DOI	10.1093/jxb/erp331
通讯作者	Mao, Xinguo
英文摘要	Osmotic stresses such as drought, salinity, and cold are major environmental factors that limit agricultural productivity worldwide. Protein phosphorylation/dephosphorylation are major signalling events induced by osmotic stress in higher plants. Sucrose non-fermenting 1-related protein kinase2 family members play essential roles in response to hyperosmotic stresses in Arabidopsis, rice, and maize. In this study, the function of TaSnRK2.4 in drought, salt, and freezing stresses in Arabidopsis was characterized. A translational fusion protein of TaSnRK2.4 with green fluorescent protein showed subcellular localization in the cell membrane, cytoplasm, and nucleus. To examine the role of TaSnRK2.4 under various environmental stresses, transgenic Arabidopsis plants overexpressing wheat TaSnRK2.4 under control of the cauliflower mosaic virus 35S promoter were generated. Overexpression of TaSnRK2.4 resulted in delayed seedling establishment, longer primary roots, and higher yield under normal growing conditions. Transgenic Arabidopsis overexpressing TaSnRK2.4 had enhanced tolerance to drought, salt, and freezing stresses, which were simultaneously supported by physiological results, including decreased rate of water loss, enhanced higher relative water content, strengthened cell membrane stability, improved photosynthesis potential, and significantly increased osmotic potential. The results show that TaSnRK2.4 is involved in the regulation of enhanced osmotic potential, growth, and development under both normal and stress conditions, and imply that TaSnRK2.4 is a multifunctional regulatory factor in Arabidopsis. Since the overexpression of TaSnRK2.4 can significantly strengthen tolerance to drought, salt, and freezing stresses and does not retard the growth of transgenic Arabidopsis plants under well-watered conditions, TaSnRK2.4 could be utilized in transgenic breeding to improve abiotic stresses in crops.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000274345000007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5438]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.NW N&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China 3.Sichuan Agr Univ, Coll Agron, Yaan 625014, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Mao, Xinguo,Zhang, Hongying,Tian, Shanjun,et al. TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis[J]. JOURNAL OF EXPERIMENTAL BOTANY,2010,61(3):683-696.
APA	Mao, Xinguo,Zhang, Hongying,Tian, Shanjun,Chang, Xiaoping,&Jing, Ruilian.(2010).TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis.JOURNAL OF EXPERIMENTAL BOTANY,61(3),683-696.
MLA	Mao, Xinguo,et al."TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis".JOURNAL OF EXPERIMENTAL BOTANY 61.3(2010):683-696.