Transcriptome analysis of grain-filling caryopses reveals involvement of multiple regulatory pathways in chalky grain formation in rice

doi:10.1186/1471-2164-11-730

	Transcriptome analysis of grain-filling caryopses reveals involvement of multiple regulatory pathways in chalky grain formation in rice
	Liu, Xiaolu 1; Guo, Tao 2; Wan, Xiangyuan 1; Wang, Haiyang 1; Zhu, Mingzhu 1; Li, Aili 1; Su, Ning 1; Shen, Yingyue 2; Mao, Bigang 1; Zhai, Huqu
刊名	BMC GENOMICS
	2010
卷号	11 页码:-
ISSN号	1471-2164
DOI	10.1186/1471-2164-11-730
通讯作者	Liu, Xiaolu
英文摘要	Background: Grain endosperm chalkiness of rice is a varietal characteristic that negatively affects not only the appearance and milling properties but also the cooking texture and palatability of cooked rice. However, grain chalkiness is a complex quantitative genetic trait and the molecular mechanisms underlying its formation are poorly understood.Results: A near-isogenic line CSSL50-1 with high chalkiness was compared with its normal parental line Asominori for grain endosperm chalkiness. Physico-biochemical analyses of ripened grains showed that, compared with Asominori, CSSL50-1 contains higher levels of amylose and 8 DP (degree of polymerization) short-chain amylopectin, but lower medium length 12 DP amylopectin. Transcriptome analysis of 15 DAF (day after flowering) caryopses of the isogenic lines identified 623 differential expressed genes (P < 0.01), among which 324 genes are up-regulated and 299 down-regulated. These genes were classified into 18 major categories, with 65.3% of them belong to six major functional groups: signal transduction, cell rescue/defense, transcription, protein degradation, carbohydrate metabolism and redox homeostasis. Detailed pathway dissection demonstrated that genes involved in sucrose and starch synthesis are up-regulated, whereas those involved in non-starch polysaccharides are down regulated. Several genes involved in oxidoreductive homeostasis were found to have higher expression levels in CSSL50-1 as well, suggesting potential roles of ROS in grain chalkiness formation.Conclusion: Extensive gene expression changes were detected during rice grain chalkiness formation. Over half of these differentially expressed genes are implicated in several important categories of genes, including signal transduction, transcription, carbohydrate metabolism and redox homeostasis, suggesting that chalkiness formation involves multiple metabolic and regulatory pathways.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; GENETICS & HEREDITY
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000286424700001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5376]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiaolu,Guo, Tao,Wan, Xiangyuan,et al. Transcriptome analysis of grain-filling caryopses reveals involvement of multiple regulatory pathways in chalky grain formation in rice[J]. BMC GENOMICS,2010,11:-.
APA	Liu, Xiaolu.,Guo, Tao.,Wan, Xiangyuan.,Wang, Haiyang.,Zhu, Mingzhu.,...&Wan, Jianmin.(2010).Transcriptome analysis of grain-filling caryopses reveals involvement of multiple regulatory pathways in chalky grain formation in rice.BMC GENOMICS,11,-.
MLA	Liu, Xiaolu,et al."Transcriptome analysis of grain-filling caryopses reveals involvement of multiple regulatory pathways in chalky grain formation in rice".BMC GENOMICS 11(2010):-.