Comparative Transcriptome Profiling Reveals Different Expression Patterns in Xanthomonas oryzae pv. oryzae Strains with Putative Virulence-Relevant Genes

doi:10.1371/journal.pone.0064267

	Comparative Transcriptome Profiling Reveals Different Expression Patterns in Xanthomonas oryzae pv. oryzae Strains with Putative Virulence-Relevant Genes
	Zhang, Fan 1; Du, Zhenglin 3; Huang, Liyu 1; Vera Cruz, Casiana 2; Zhou, Yongli 1; Li, Zhikang 1
刊名	PLOS ONE
	2013
卷号	8 期号:5 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0064267
通讯作者	Zhang, Fan
英文摘要	Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of rice bacterial blight, which is a major rice disease in tropical Asian countries. An attempt has been made to investigate gene expression patterns of three Xoo strains on the minimal medium XOM2, PXO99 (P6) and PXO86 (P2) from the Philippines, and GD1358 (C5) from China, which exhibited different virulence in 30 rice varieties, with putative virulence factors using deep sequencing. In total, 4,781 transcripts were identified in this study, and 1,151 and 3,076 genes were differentially expressed when P6 was compared with P2 and with C5, respectively. Our results indicated that Xoo strains from different regions exhibited distinctly different expression patterns of putative virulence-relevant genes. Interestingly, 40 and 44 genes involved in chemotaxis and motility exhibited higher transcript alterations in C5 compared with P6 and P2, respectively. Most other genes associated with virulence, including exopolysaccharide (EPS) synthesis, Hrp genes and type III effectors, including Xanthomonas outer protein (Xop) effectors and transcription activator-like (TAL) effectors, were down-regulated in C5 compared with P6 and P2. The data were confirmed by real-time quantitative RT-PCR, tests of bacterial motility, and enzyme activity analysis of EPS and xylanase. These results highlight the complexity of Xoo and offer new avenues for improving our understanding of Xoo-rice interactions and the evolution of Xoo virulence.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000319725500075
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5040]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China 2.Int Rice Res Inst, Manila, Philippines 3.Chinese Acad Sci, Beijing Inst Genom, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Fan,Du, Zhenglin,Huang, Liyu,et al. Comparative Transcriptome Profiling Reveals Different Expression Patterns in Xanthomonas oryzae pv. oryzae Strains with Putative Virulence-Relevant Genes[J]. PLOS ONE,2013,8(5):-.
APA	Zhang, Fan,Du, Zhenglin,Huang, Liyu,Vera Cruz, Casiana,Zhou, Yongli,&Li, Zhikang.(2013).Comparative Transcriptome Profiling Reveals Different Expression Patterns in Xanthomonas oryzae pv. oryzae Strains with Putative Virulence-Relevant Genes.PLOS ONE,8(5),-.
MLA	Zhang, Fan,et al."Comparative Transcriptome Profiling Reveals Different Expression Patterns in Xanthomonas oryzae pv. oryzae Strains with Putative Virulence-Relevant Genes".PLOS ONE 8.5(2013):-.