Roles of NIA/NR/NOA1-dependent nitric oxide production and HY1 expression in the modulation of Arabidopsis salt tolerance

doi:10.1093/jxb/ert149

	Roles of NIA/NR/NOA1-dependent nitric oxide production and HY1 expression in the modulation of Arabidopsis salt tolerance
	Xie, Yanjie 1; Mao, Yu 1; Lai, Diwen 1; Zhang, Wei 1; Zheng, Tianqing 2; Shen, Wenbiao 1
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2013
卷号	64 期号:10 页码:3045-3060
关键词	Arabidopsis haem oxygenase-1 (HY1) NIA NR nitric oxide NOA1 salt tolerance
ISSN号	0022-0957
DOI	10.1093/jxb/ert149
通讯作者	Xie, Yanjie
英文摘要	Despite substantial evidence on the separate roles of Arabidopsis nitric oxide-associated 1 (NOA1)-associated nitric oxide (NO) production and haem oxygenase 1 (HY1) expression in salt tolerance, their integrative signalling pathway remains largely unknown. To fill this knowledge gap, the interaction network among nitrate reductase (NIA/NR)- and NOA1-dependent NO production and HY1 expression was studied at the genetic and molecular levels. Upon salinity stress, the majority of NO production was attributed to NIA/NR/NOA1. Further evidence confirmed that HY1 mutant hy1-100, nia1/2/noa1, and nia1/2/noa1/hy1-100 mutants exhibited progressive salt hypersensitivity, all of which were significantly rescued by three NO-releasing compounds. The salinity-tolerant phenotype and the stronger NO production in gain-of-function mutant of HY1 were also blocked by the NO synthetic inhibitor and scavenger. Although NO- or HY1-deficient mutants showed a compensatory mode of upregulation of HY1 or slightly increased NO production, respectively, during 2 d of salt treatment, downregulation of ZAT10/12-mediated antioxidant gene expression (cAPX1/2 and FSD1) was observed after 7 d of treatment. The hypersensitive phenotypes and stress-related genes expression profiles were differentially rescued or blocked by the application of NO- (in particular) or carbon monoxide (CO)-releasing compounds, showing a synergistic mode. Similar reciprocal responses were observed in the nia1/2/noa1/hy1-100 quadruple mutant, with the NO-releasing compounds exhibit the maximal rescuing responses. Overall, the findings present the combination of compensatory and synergistic modes, linking NIA/NR/NOA1-dependent NO production and HY1 expression in the modulation of plant salt tolerance.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000321055700031
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5030]
专题	作物科学研究所_分子生物学系
作者单位	1.Nanjing Agr Univ, Coll Life Sci, Co Lab Nanjing Agr Univ & Carl Zeiss Far East, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Xie, Yanjie,Mao, Yu,Lai, Diwen,et al. Roles of NIA/NR/NOA1-dependent nitric oxide production and HY1 expression in the modulation of Arabidopsis salt tolerance[J]. JOURNAL OF EXPERIMENTAL BOTANY,2013,64(10):3045-3060.
APA	Xie, Yanjie,Mao, Yu,Lai, Diwen,Zhang, Wei,Zheng, Tianqing,&Shen, Wenbiao.(2013).Roles of NIA/NR/NOA1-dependent nitric oxide production and HY1 expression in the modulation of Arabidopsis salt tolerance.JOURNAL OF EXPERIMENTAL BOTANY,64(10),3045-3060.
MLA	Xie, Yanjie,et al."Roles of NIA/NR/NOA1-dependent nitric oxide production and HY1 expression in the modulation of Arabidopsis salt tolerance".JOURNAL OF EXPERIMENTAL BOTANY 64.10(2013):3045-3060.