A Role for a Dioxygenase in Auxin Metabolism and Reproductive Development in Rice

doi:10.1016/j.devcel.2013.09.005

	A Role for a Dioxygenase in Auxin Metabolism and Reproductive Development in Rice
	Zhao, Zhigang 1; Zhang, Yunhui 1; Liu, Xi 1; Zhang, Xin 2; Liu, Shichang 3; Yu, Xiaowen 1; Ren, Yulong 1; Zheng, Xiaomin 2; Zhou, Kunneng 1; Jiang, Ling 1
刊名	DEVELOPMENTAL CELL
	2013
卷号	27 期号:1 页码:113-122
ISSN号	1534-5807
DOI	10.1016/j.devcel.2013.09.005
通讯作者	Zhao, Zhigang
英文摘要	Indole-3-acetic acid (IAA), the natural auxin in plants, regulates many aspects of plant growth and development. Extensive analyses have elucidated the components of auxin biosynthesis, transport, and signaling, but the physiological roles and molecular mechanisms of auxin degradation remain elusive. Here, we demonstrate that the dioxygenase for auxin oxidation (DAO) gene, encoding a putative 2-oxoglutarate-dependent-Fe (II) dioxygenase, is essential for anther dehiscence, pollen fertility, and seed initiation in rice. Rice mutant lines lacking a functional DAO display increased levels of free IAA in anthers and ovaries. Furthermore, exogenous application of IAA or overexpression of the auxin biosynthesis gene OsYUCCA1 phenocopies the dao mutants. We show that recombinant DAO converts the active IAA into biologically inactive 2-oxoindole-3-acetic acid (OxIAA) in vitro. Collectively, these data support a key role of DAO in auxin catabolism and maintenance of auxin homeostasis central to plant reproductive development.
学科主题	Cell Biology ; Developmental Biology ; CELL BIOLOGY ; DEVELOPMENTAL BIOLOGY
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000326138800011
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4994]
专题	作物科学研究所_分子生物学系
作者单位	1.Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 3.Beijing Forestry Univ, Coll Life Sci & Biotechnol, Beijing 100083, Peoples R China 4.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Zhigang,Zhang, Yunhui,Liu, Xi,et al. A Role for a Dioxygenase in Auxin Metabolism and Reproductive Development in Rice[J]. DEVELOPMENTAL CELL,2013,27(1):113-122.
APA	Zhao, Zhigang.,Zhang, Yunhui.,Liu, Xi.,Zhang, Xin.,Liu, Shichang.,...&Wan, Jianmin.(2013).A Role for a Dioxygenase in Auxin Metabolism and Reproductive Development in Rice.DEVELOPMENTAL CELL,27(1),113-122.
MLA	Zhao, Zhigang,et al."A Role for a Dioxygenase in Auxin Metabolism and Reproductive Development in Rice".DEVELOPMENTAL CELL 27.1(2013):113-122.