A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice

doi:10.1007/s00299-013-1498-y

	A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice
	Chen, Hong 1; Cheng, Zhijun 2; Ma, Xiaoding 2; Wu, Han 1; Liu, Yanling 1; Zhou, Kunneng 1; Chen, Yilin 1; Ma, Weiwei 2; Bi, Jingcui 2; Zhang, Xin 2
刊名	PLANT CELL REPORTS
	2013
卷号	32 期号:12 页码:1855-1867
关键词	Heme oxygenase-1 Map-based cloning Yellow-green leaves Chloroplast Chl biosynthesis Rice
ISSN号	0721-7714
DOI	10.1007/s00299-013-1498-y
通讯作者	Chen, Hong
英文摘要	An insert mutation of YELLOW - GREEN LEAF2 , encoding Heme Oxygenase 1 , results in significant reduction of its transcript levels, and therefore impairs chlorophyll biosynthesis in rice.Heme oxygenase (HO) in higher plants catalyzes the degradation of heme to synthesize phytochrome precursor and its roles conferring the photoperiodic control of flowering in rice have been revealed. However, its involvement in regulating rice chlorophyll (Chl) synthesis is not fully explored. In this study, we isolated a rice mutant named yellow-green leaf 2 (ygl2) from a Co-60-irradiated population. Normal grown ygl2 seedlings showed yellow-green leaves with reduced contents of Chl and tetrapyrrole intermediates whereas an increase of Chl a/b ratio. Ultrastructural analyses demonstrated grana were poorly stacked in ygl2 mutant, resulting in underdevelopment of chloroplasts. The ygl2 locus was mapped to chromosome 6 and isolated via map-based cloning. Sequence analysis indicated that it encodes the rice HO1 and its identity was verified by transgenic complementation test and RNA interference. A 7-Kb insertion was found in the first exon of YGL2/HO1, resulting in significant reduction of YGL2 expressions in the ygl2 mutant. YGL2 was constitutively expressed in a variety of rice tissues with the highest levels in leaves and regulated by temperature. In addition, we found expression levels of some genes associated with Chl biosynthesis and photosynthesis were concurrently altered in ygl2 mutant. These results provide direct evidence that YGL2 has a vital function in rice Chl biosynthesis.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000327218700003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4975]
专题	作物科学研究所_分子生物学系
作者单位	1.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Chen, Hong,Cheng, Zhijun,Ma, Xiaoding,et al. A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice[J]. PLANT CELL REPORTS,2013,32(12):1855-1867.
APA	Chen, Hong.,Cheng, Zhijun.,Ma, Xiaoding.,Wu, Han.,Liu, Yanling.,...&Jiang, Ling.(2013).A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice.PLANT CELL REPORTS,32(12),1855-1867.
MLA	Chen, Hong,et al."A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice".PLANT CELL REPORTS 32.12(2013):1855-1867.