Molecular characterisation of high molecular weight glutenin allele Glu-B1 h encoding 1Bx14+1By15 subunits in bread wheat (Triticum aestivum L.)

doi:10.1071/CP13409

	Molecular characterisation of high molecular weight glutenin allele Glu-B1 h encoding 1Bx14+1By15 subunits in bread wheat (Triticum aestivum L.)
	Xiao, Lele 1; Wang, Ke 2; Liu, Yanlin 1; Ye, Xingguo 2; Ma, Wujun 3; Yan, Yueming 1
刊名	CROP & PASTURE SCIENCE
	2014
卷号	65 期号:3 页码:215-226
关键词	Glu-B1h, HMW-GS, molecular cloning, SNPs, wheat.
ISSN号	1836-0947
DOI	10.1071/CP13409
通讯作者	Xiao, Lele
英文摘要	In this study, the authentic high molecular weight glutenin (HMW-GS) allele Glu-B1 h encoding for subunits 1Bx14 and 1By15 from German bread wheat cultivars Hanno and Imbros was identified and cross-verified by a suite of established protein analysis technologies, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis, reversed-phase high-performance liquid chromatography, reversed-phase ultra-performance liquid chromatography, and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS). The complete encoding sequences were isolated by allele-specific PCR, and consist of 2367 bp for 1Bx14 and 2151 bp for 1By15 and encode 789 and 717 amino acid residues, respectively. The deduced molecular masses of two subunit genes were 82 340.13 Da and 74 736.13 Da, corresponding well to those determined by MALDI-TOF-MS. The presence and authenticity of 1Bx14 and 1By15 subunits were further confirmed by liquid chromatography coupled to tandem mass spectrometry and heterologous expression in E. coli. Comparative analysis demonstrated that 1Bx14 possessed one deletion and 20 single-nucleotide polymorphism variations compared with seven other Glu-B1 x-type HMW-GS genes that mainly resulted from C-T substitutions, whereas compared with five other Glu-B1 y-type HMW-GS genes, 1By15 displayed few variations. Phylogenetic analysis based on the complete coding sequences of the published HMW-GS genes showed that 1Bx14 had a high divergence with other 1Bx subunit genes, whereas 1By15 displayed greater similarity with 1By20. A possible evolutionary route for 1Bx14 gene formation is proposed, which might have resulted from an intra-strand illegitimate recombination event that occurred ~1.32 million years ago.
学科主题	Agriculture, Multidisciplinary ; AGRICULTURE, MULTIDISCIPLINARY
语种	英语
出版者	CSIRO PUBLISHING
WOS记录号	WOS:000334269300001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4963]
专题	作物科学研究所_分子生物学系
作者单位	1.Capital Normal Univ, Key Lab Genet & Biotechnol, Coll Life Sci, Beijing 100048, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 3.Murdoch Univ, Australia China Wheat Improvement Ctr, Australian Export Grains Innovat Ctr, Perth, WA 6150, Australia
推荐引用方式 GB/T 7714	Xiao, Lele,Wang, Ke,Liu, Yanlin,et al. Molecular characterisation of high molecular weight glutenin allele Glu-B1 h encoding 1Bx14+1By15 subunits in bread wheat (Triticum aestivum L.)[J]. CROP & PASTURE SCIENCE,2014,65(3):215-226.
APA	Xiao, Lele,Wang, Ke,Liu, Yanlin,Ye, Xingguo,Ma, Wujun,&Yan, Yueming.(2014).Molecular characterisation of high molecular weight glutenin allele Glu-B1 h encoding 1Bx14+1By15 subunits in bread wheat (Triticum aestivum L.).CROP & PASTURE SCIENCE,65(3),215-226.
MLA	Xiao, Lele,et al."Molecular characterisation of high molecular weight glutenin allele Glu-B1 h encoding 1Bx14+1By15 subunits in bread wheat (Triticum aestivum L.)".CROP & PASTURE SCIENCE 65.3(2014):215-226.