Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1

doi:10.1016/j.plantsci.2013.11.008

	Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1
	Gao, Ying 1; Zan, Xin-Li 1,2; Wu, Xue-Feng 1; Yao, Lei 3; Chen, Yu-Ling 2; Jia, Shuang-Wei 1; Zhao, Kai-Jun 1
刊名	PLANT SCIENCE
	2014
卷号	215 页码:190-198
关键词	Inducible promoter cis-Acting element Fungal elicitor Transient expression W-box-like
ISSN号	0168-9452
DOI	10.1016/j.plantsci.2013.11.008
通讯作者	Gao, Ying
英文摘要	Chitinases are a group of pathogenesis-related proteins. The Brassica juncea chitinase gene BjCHI1 is highly inducible by pathogenic fungal infection, suggesting that the promoter of BjCHI1 might contain specific cis-acting element responsive to fungal attack. To identify the fungus-responsive element in BjCHI1 promoter (BjC-P), a series of binary plant transformation vectors were constructed by fusing the BjC-P or its deletion-derivatives to p-glucuronidase (GUS) reporter gene. Expression of the GUS reporter gene was systematically assayed by a transient gene expression system in Nicotiana benthamiana leaves treated with fungal elicitor Hexa-N-Acetyl-Chitohexaose, as well as in transgenic Arabidopsis plants inoculated with fungus Botrytis cinerea. The histochemical and quantitative GUS assays showed that the W-boxlike element (GTAGTGACTCAT) in the region (-668 to -657) was necessary for the fungus-response, although there were another five W-box-like elements in BjC-P. In addition, gain-of-function analysis demonstrated that the fragment (-409 to -337) coupled to the W-box-like element was needed for full magnitude of the fungal induction. These results revealed the existence of a novel regulation mechanism of W-box-like element involved in plant pathogenic resistance, and will benefit the potential application of BjC-P in engineering crops. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; PLANT SCIENCES
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000330154000023
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4932]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Genet & Breeding, Minist Agr,Natl Key Facil Crop Gene Resources & G, Beijing 100081, Peoples R China 2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050024, Heibei, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Beijing Agr Biotechnol Res Ctr, Beijing 100097, Peoples R China
推荐引用方式 GB/T 7714	Gao, Ying,Zan, Xin-Li,Wu, Xue-Feng,et al. Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1[J]. PLANT SCIENCE,2014,215:190-198.
APA	Gao, Ying.,Zan, Xin-Li.,Wu, Xue-Feng.,Yao, Lei.,Chen, Yu-Ling.,...&Zhao, Kai-Jun.(2014).Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1.PLANT SCIENCE,215,190-198.
MLA	Gao, Ying,et al."Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1".PLANT SCIENCE 215(2014):190-198.