The rice OsDG2 encoding a glycine-rich protein is involved in the regulation of chloroplast development during early seedling stage

doi:10.1007/s00299-013-1549-4

	The rice OsDG2 encoding a glycine-rich protein is involved in the regulation of chloroplast development during early seedling stage
	Jiang, Quan 1; Ma, XiaoJing 1; Gong, XiaoDi 1; Zhang, JianHui 1,2; Teng, Sheng 2; Xu, JianLong 3; Lin, DongZhi 1; Dong, YanJun 1
刊名	PLANT CELL REPORTS
	2014
卷号	33 期号:5 页码:733-744
关键词	Rice Chloroplast development Chlorophyll content Glycine-rich proteins (GRPs) Delayed greening
ISSN号	0721-7714
DOI	10.1007/s00299-013-1549-4
通讯作者	Jiang, Quan
英文摘要	OsDG2 gene encoded a novel chloroplast-targeted GRP in rice. Disruption of the OsDG2 would lead to delayed greening phenotype and affected expression levels of genes associated with chloroplast development at early leaf stage of rice.Glycine-rich proteins (GRPs) participate in various biological processes in plants. However, the evidence of GRPs involved in chloroplast development in plants is quite limited. In this study, we identified a rice GRP gene mutant named osdg2 (O ryza s ativa d elayed g reening 2), which exhibits delayed greening phenotype characterized as bright yellow leaves before the three-leaf stage and thereafter turns to normal green. Further study showed that the mutant phenotype was consistent with changes in chlorophyll content and chloroplast development. The rice OsDG2 gene, encoding a novel GRP protein, was located on chromosome 2 through map-based cloning method and confirmed by molecular complementation tests. Subcellular localization results showed that OsDG2 was targeted in chloroplasts. In addition, the OsDG2 transcripts were highly expressed in leaves and undetectable in other tissues, showing the tissue-specific expression. In osdg2 mutant, the expression levels of most genes associated with chloroplast development were severely decreased in the 3rd leaves, but almost recovered to wild-type level in the 4th leaves. Our findings indicated that the nuclear-encoded OsDG2 plays important roles in chloroplast development at early leaf stage of rice.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000334514300006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4891]
专题	作物科学研究所_分子生物学系
作者单位	1.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Quan,Ma, XiaoJing,Gong, XiaoDi,et al. The rice OsDG2 encoding a glycine-rich protein is involved in the regulation of chloroplast development during early seedling stage[J]. PLANT CELL REPORTS,2014,33(5):733-744.
APA	Jiang, Quan.,Ma, XiaoJing.,Gong, XiaoDi.,Zhang, JianHui.,Teng, Sheng.,...&Dong, YanJun.(2014).The rice OsDG2 encoding a glycine-rich protein is involved in the regulation of chloroplast development during early seedling stage.PLANT CELL REPORTS,33(5),733-744.
MLA	Jiang, Quan,et al."The rice OsDG2 encoding a glycine-rich protein is involved in the regulation of chloroplast development during early seedling stage".PLANT CELL REPORTS 33.5(2014):733-744.