Comparative Metabolite Profiling of Two Rice Genotypes with Contrasting Salt Stress Tolerance at the Seedling Stage

doi:10.1371/journal.pone.0108020

	Comparative Metabolite Profiling of Two Rice Genotypes with Contrasting Salt Stress Tolerance at the Seedling Stage
	Zhao, Xiuqin; Wang, Wensheng; Zhang, Fan; Deng, Jianli; Li, Zhikang; Fu, Binying
刊名	PLOS ONE
	2014
卷号	9 期号:9 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0108020
通讯作者	Zhao, Xiuqin
英文摘要	Background: Rice is sensitive to salt stress, especially at the seedling stage, with rice varieties differing remarkably in salt tolerance (ST). To understand the physiological mechanisms of ST, we investigated salt stress responses at the metabolite level.Methods: Gas chromatography-mass spectrometry was used to profile metabolite changes in the salt-tolerant line FL478 and the sensitive variety IR64 under a salt-stress time series. Additionally, several physiological traits related to ST were investigated.Results: We characterized 92 primary metabolites in the leaves and roots of the two genotypes under stress and control conditions. The metabolites were temporally, tissue-specifically and genotype-dependently regulated under salt stress. Sugars and amino acids (AAs) increased significantly in the leaves and roots of both genotypes, while organic acids (OAs) increased in roots and decreased in leaves. Compared with IR64, FL478 experienced greater increases in sugars and AAs and more pronounced decreases in OAs in both tissues; additionally, the maximum change in sugars and AAs occurred later, while OAs changed earlier. Moreover, less Na+ and higher relative water content were observed in FL478. Eleven metabolites, including AAs and sugars, were specifically increased in FL478 over the course of the treatment.Conclusions: Metabolic responses of rice to salt stress are dynamic and involve many metabolites. The greater ST of FL478 is due to different adaptive reactions at different stress times. At early salt-stress stages, FL478 adapts to stress by decreasing OA levels or by quickly depressing growth; during later stages, more metabolites are accumulated, thereby serving as compatible solutes against osmotic challenge induced by salt stress.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000345745400039
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4836]
专题	作物科学研究所_分子生物学系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xiuqin,Wang, Wensheng,Zhang, Fan,et al. Comparative Metabolite Profiling of Two Rice Genotypes with Contrasting Salt Stress Tolerance at the Seedling Stage[J]. PLOS ONE,2014,9(9):-.
APA	Zhao, Xiuqin,Wang, Wensheng,Zhang, Fan,Deng, Jianli,Li, Zhikang,&Fu, Binying.(2014).Comparative Metabolite Profiling of Two Rice Genotypes with Contrasting Salt Stress Tolerance at the Seedling Stage.PLOS ONE,9(9),-.
MLA	Zhao, Xiuqin,et al."Comparative Metabolite Profiling of Two Rice Genotypes with Contrasting Salt Stress Tolerance at the Seedling Stage".PLOS ONE 9.9(2014):-.