Overexpression of Folate Biosynthesis Genes in Rice (Oryza sativa L.) and Evaluation of Their Impact on Seed Folate Content

doi:10.1007/s11130-014-0450-9

	Overexpression of Folate Biosynthesis Genes in Rice (Oryza sativa L.) and Evaluation of Their Impact on Seed Folate Content
	Dong, Wei; Cheng, Zhi-jun; Lei, Cai-lin; Wang, Xiao-le; Wang, Jiu-lin; Wang, Jie; Wu, Fu-qing; Zhang, Xin; Guo, Xiu-ping; Zhai, Hu-qu
刊名	PLANT FOODS FOR HUMAN NUTRITION
	2014
卷号	69 期号:4 页码:379-385
关键词	Staple food-folate biofortification-metabolic engineering-gene stacking
ISSN号	0921-9668
DOI	10.1007/s11130-014-0450-9
通讯作者	Dong, Wei
英文摘要	Folate (vitamin B9) deficiency is a global health problem especially in developing countries where the major staple foods such as rice contain extremely low folates. Biofortification of rice could be an alternative complement way to fight folate deficiency. In this study, we evaluated the availability of the genes in each step of folate biosynthesis pathway for rice folate enhancement in the japonica variety kitaake genetic background. The first enzymes GTP cyclohydrolase I (GTPCHI) and aminodeoxychorismate synthase (ADCS) in the pterin and para-aminobenzoate branches resulted in significant increase in seed folate content, respectively (P < 0.01). Overexpression of two closely related enzymes dihydrofolate synthase (DHFS) and folypolyglutamate synthase (FPGS), which perform the first and further additions of glutamates, produced slightly increase in seed folate content separately. The GTPCHI transgene was combined with each of the other transgenes except ADCS to investigate the effects of gene stacking on seed folate accumulation. Seed folate contents in the gene-stacked plants were higher than the individual low-folate transgenic parents, but lower than the high-folate GTPCHI transgenic lines, pointing to an inadequate supply of para-aminobenzoic acid (PABA) precursor initiated by ADCS in constraining folate overproduction in gene-stacked plants.
学科主题	Plant Sciences ; Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000346058300014
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4804]
专题	作物科学研究所_分子生物学系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Dong, Wei,Cheng, Zhi-jun,Lei, Cai-lin,et al. Overexpression of Folate Biosynthesis Genes in Rice (Oryza sativa L.) and Evaluation of Their Impact on Seed Folate Content[J]. PLANT FOODS FOR HUMAN NUTRITION,2014,69(4):379-385.
APA	Dong, Wei.,Cheng, Zhi-jun.,Lei, Cai-lin.,Wang, Xiao-le.,Wang, Jiu-lin.,...&Wan, Jian-min.(2014).Overexpression of Folate Biosynthesis Genes in Rice (Oryza sativa L.) and Evaluation of Their Impact on Seed Folate Content.PLANT FOODS FOR HUMAN NUTRITION,69(4),379-385.
MLA	Dong, Wei,et al."Overexpression of Folate Biosynthesis Genes in Rice (Oryza sativa L.) and Evaluation of Their Impact on Seed Folate Content".PLANT FOODS FOR HUMAN NUTRITION 69.4(2014):379-385.