Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis

doi:10.1016/S2095-3119(14)60872-8

	Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis
	Zhang Fan 1,2; Chen Xun-Ji 2,3; Wang Jian-Hua 1; Zheng Jun 2
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2015
卷号	14 期号:7 页码:1229-1241
关键词	maize salt and drought stresses ZmSnRK2.11 SnRK2
ISSN号	2095-3119
DOI	10.1016/S2095-3119(14)60872-8
通讯作者	Zhang Fan
英文摘要	Sucrose non-fermenting-1 related protein kinase 2 (SnRK2) is a unique family of protein kinases associated with abiotic stress signal transduction in plants. In this study, a maize SnRK2 gene ZmSnRK2.11 was cloned and characterized. The results showed that ZmSnRK2.11 is up-regulated by high-salinity and dehydration treatment, and it is expressed mainly in maize mature leaf. A transient expression assay using onion epidermal cells revealed that ZmSnRK2.11-GFP fusion proteins are localized to both the nucleus and cytoplasm. Overexpressing-ZmSnRK2.11 in Arabidopsis resulted in salt and drought sensitivity phenotypes that exhibited an increased rate of water loss, reduced relative water content, delayed stoma closure, accumulated less free proline content and increased malondialdehyde (MDA) content relative to the phenotypes observed in wild-type (WT) control. Furthermore, overexpression of ZmSnRK2.11 up-regulated the expression of the genes ABI1 and ABI2 and decreased the expression of DREB2A and P5CS1. Taken together, our results suggest that ZmSnRK2.11 is a possible negative regulator involved in the salt and drought stress signal transduction pathways in plants.
学科主题	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000358188200002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4781]
专题	作物科学研究所_分子生物学系
作者单位	1.China Agr Univ, Coll Agr & Biotechnol, Beijing 100193, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 3.Xinjiang Acad Agr Sci, Inst Nucl Technol & Biotechnol, Urumqi 830091, Peoples R China
推荐引用方式 GB/T 7714	Zhang Fan,Chen Xun-Ji,Wang Jian-Hua,et al. Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2015,14(7):1229-1241.
APA	Zhang Fan,Chen Xun-Ji,Wang Jian-Hua,&Zheng Jun.(2015).Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis.JOURNAL OF INTEGRATIVE AGRICULTURE,14(7),1229-1241.
MLA	Zhang Fan,et al."Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis".JOURNAL OF INTEGRATIVE AGRICULTURE 14.7(2015):1229-1241.